Биохимия

Description

Издание содержит рекомендации по подготовке к лабораторно-практическим занятиям по биологической химии. По каждой теме даны: цель занятия, вопросы для обсуждения, литература для подготовки, описание лабораторных работ и их клинико-диагностическое значение. Включены вопросы для подготовки к коллоквиумам, примерный перечень экзаменационных вопросов. Предназначено для иностранных студентов 2-го курса, обучающихся на английском языке. БИОХИМИЯ PRACTICAL BIOCHEMISTRY Contents 1. Topic: Introduction to practical work. Introduction to biochemistry. The structure of amino acids and peptides. Determination of the protein content in biological fluids 2. Topic: The structure and functions of proteins. Protein hydrolysis 3. Topic: The three-dimensional structure of proteins. Physical and chemical properties of proteins. Mechanisms of proteins sedimentation 4. Topic: Methods of separation, isolation and purification of proteins. Compound proteins 5. Topic: Enzymes. Classification, structure, properties 6. Topic: Regulation of enzyme activity. Determination of enzyme activity 7. Colloquium: “The chemistry of proteins, enzymes” 8. Topic: Introduction to metabolism. Central metabolic pathways (oxidative decarboxilation of pyruvate. Citric acid cycle) 9. Topic: Biological oxidation. Pathways of oxygen utilization by cells. Oxidative phosphorYlation 10. Topic: Digestion of carbohydrates. Glycogenesis and glycogenolysis. Glycolysis 11. Topic: Metabolic pathways of pyruvate. Gluconeogenesis. Aerobic oxidation of glucose to end-products (CO2 AND H2O). Determination of blood glucose 12. Topic: Secondary pathways of glucose metabolism. Effect of hormones on the blood glucose level 13. Colloquium: “Introduction to metabolism, central metabolic pathways, biological oxidation, oxidative phosphorylation, carbohydrate metabolism” 14. Topic: Lipid metabolism. Digestion and re-synthesis. Transport of exogenous lipids. Evaluation of lipase activity 15. Topic: Lipid transport in blood. Cholesterol metabolism. Storage and mobilization of lipids. Determination of plasma β-lipoproteins 16. Topic: Intracellular metabolism of fatty ACIDS. Ketone bodies. Determination of cholesterol in serum 17. Colloquium: “Lipid metabolism” 18. Topic: Control over practical skills of biochemical analysis 19. Topic: Digestion and absorption of proteins. Analysis of gastric juice 20. Topic: Intracellular amino acid metabolism. Determination of amino transferase activity in serum 21. Topic: Detoxification of ammonia. Determination of nonprotein nitrogen in blood and urea in urine 22. Topic: Nucleoproteins chemistry and metabolism. Determination of uric acid in urine 23. Topic (seminar): Matrix biosyntheses (synthesis of dna, rna, proteins) 24. Colloquium: “Metabolism of simple proteins and nucleoproteins. Synthesis of dna, rna and proteins” 25. Topic: Hormones, general characteristic and peculiarities of biological action. Qualitative reactions for hormones 26. Topic: Biochemistry of hormones. Glucose tolerance test 27. Topic: Physical and chemical properties of the blood. Hemoglobinoses 28. Topic: Blood plasma proteins. Blood clotting system 29. Topic: Liver biochemistry 30. Colloquium: “Hormones, biochemistry of the liver, biochemistry of the blood” 31. Topic: Biochemistry of the urine. Physiological and pathological components of the urine 32. Topic: Biochemistry of nutrition. Role of proteins, fats, carbohydrates, vitamins 33. Topic: Biochemistry of nutrition. Mineral substances. regulation of water-electrolyte balance. Integration of metabolism 34. Colloquium: biochemistry of nutrition, biochemistry of urine. Integration of metabolism 35. Topic: Control over practical skills of biochemical analys is: analysis of gastric juice and urine Appendix