ENGLISH

Handbook of ELISPOT: Methods and Protocols

By Jonathon D. Sedgwick (auth.) , Alexander E. Kalyuzhny (eds.)

Book information

Publisher
Humana Press
Year
2005
ISBN
9781588294692, 1588294692, 1592599036
DOI
10.1385/1592599036
Open Library ID
OL17152114M
Language
english
Format
PDF
Filesize
6 MB (6466055 bytes)
Series
Methods in Molecular Biology 302
Edition
1
Pages
337\337
Time added
2010-08-30 15:18:36
Immunology

Description

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

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