Inflammation and Cancer: Methods and Protocols

Description

This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field. Front Matter ....Pages i-xii Front Matter ....Pages 1-1 In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer (Ka Yee Fung, Tracy Putoczki)....Pages 3-13 Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer (Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, Kate D. Sutherland)....Pages 15-29 Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer (William Berry, Daniel Croagh)....Pages 31-40 Modelling Intestinal Carcinogenesis Using In Vitro Organoid Cultures (Thierry Jardé, Genevieve Kerr, Reyhan Akhtar, Helen E. Abud)....Pages 41-52 Protocols to Evaluate Cigarette Smoke-Induced Lung Inflammation and Pathology in Mice (Ross Vlahos, Steven Bozinovski)....Pages 53-63 Tracking Competent Host Defence to Chronic Inflammation: An In Vivo Model of Peritonitis (Javier Uceda Fernandez, David Millrine, Simon A. Jones)....Pages 65-75 Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology (Catherine L. Kennedy, Elizabeth L. Hartland)....Pages 77-89 In Vivo Infection Model of Severe Influenza A Virus (Ashley Mansell, Michelle D. Tate)....Pages 91-99 In Vivo Models for Inflammatory Arthritis (Gareth W. Jones, David G. Hill, Katie Sime, Anwen S. Williams)....Pages 101-118 Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease (Le Son Tran, Richard L. Ferrero)....Pages 119-126 Dissecting Interleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer (Christoph Garbers, Stefan Rose-John)....Pages 127-140 Front Matter ....Pages 141-141 Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models (Tae-Su Han, Masanobu Oshima)....Pages 143-153 Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease (Mohamed Saad, Saleela M. Ruwanpura)....Pages 155-162 Quantifying Caspase-1 Activity in Murine Macrophages (Dave Boucher, Amy Chan, Connie Ross, Kate Schroder)....Pages 163-176 Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation (Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, Matthew P. McCormack)....Pages 177-184 Analysis of DNA Methylation in Tissues Exposed to Inflammation (Naoko Hattori, Toshikazu Ushijima)....Pages 185-199 A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens (Leonie A. Cluse, Iva Nikolic, Deborah Knight, Piyush B. Madhamshettiwar, Jennii Luu, Karla J. Cowley et al.)....Pages 201-227 Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18 (Brendan J. Jenkins, Virginie Deswaerte)....Pages 229-235 Optimization Techniques for miRNA Expression in Low Frequency Immune Cell Populations (Victoria G. Lyons, Natalie L. Payne, Claire E. McCoy)....Pages 237-256 Assessing the cGAS-cGAMP-STING Activity of Cancer Cells (Geneviève Pépin, Michael P. Gantier)....Pages 257-266 Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies (Nicholas P. D. Liau, Jeffrey J. Babon)....Pages 267-280 Production of Recombinant Killer Immunoglobulin-Like Receptors for Crystallography and Luminex-Based Assays (Phillip Pymm, Julian P. Vivian)....Pages 281-299 Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry (Florian Wiede, Tony Tiganis)....Pages 301-311 Back Matter ....Pages 313-315