Toxicogenomics

Description

The meteoric rate at which the human genome is being sequenced has presented to the research community a vast array of newly discovered genes, which in tum has catalyzed an even more dramatic effort to decipher this voluminous data set into understanding how genes function both individually and in complex pathways that regulate the biochemistry of life. A compendium of gene expression data, enhanced by complete proteomic analysis, will enable investigators to probe into the complexities of the mechanisms of normal genetic and metabolic pathways and, subsequently, how disease occurs when they malfunction. The new science of toxicogenomics combines genomic, proteomic, and informatics technologies, and biological research can now foresee a time when there will be a full comprehension of the complex dynamic mechanisms of genetics, biochemistry, and physiology. The inherent power of toxicogenomics derives from an amalgamation of multiple scientific disciplines that were originally drawn together to facilitate sequencing the three billion bases that comprise the human genome. Traditionally, the science of toxicology has been founded upon empirical codification of dose-related effects. It has evolved to studies that are directed towards understanding the mechanisms by which individual agents cause their effects in humans. Due to technical limitations, this process has been relatively slow, since it has accomplished one chemical or one effect at a time. Front Matter....Pages II-XXIII Front Matter....Pages 1-1 Introduction: Toxicogenomics — a new paradigm of toxicology....Pages 3-11 Toxicogenomics in Genome Science World....Pages 12-19 Application of transcript profiling to mechanism-based toxicology....Pages 20-28 Front Matter....Pages 29-29 Application of DNA microarrays for predicting toxicity and evaluating cross-species extrapolation....Pages 31-38 Use of reverse genetics and cDNA arrays to understand ‘dioxin’ toxicity....Pages 39-45 Front Matter....Pages 47-47 Application of gene arrays in toxicology....Pages 49-56 The ToxExpress™ Module: applying gene expression data to predictive toxicology....Pages 57-61 Taming the mass of microarray data: systematic approaches to gene expression analysis....Pages 62-69 Applications of ProteinChip® Systems in Toxicological Assessment....Pages 70-74 Front Matter....Pages 75-75 Toxicogenomics: making progress by maintaining a focus on the fundamentals of toxicology....Pages 77-81 A microarray analysis of differential gene expression associated with the development of doxorubicin resistance in breast carcinoma....Pages 82-87 Screening trial of cancer chemopreventive agent by using combination of DNA chip technology and Cell Signaling Network DataBase (CSNDB)....Pages 88-99 Application of methacarn fixation for genetic analysis in microdissected paraffin-embedded tissue specimens....Pages 100-106 Front Matter....Pages 107-107 ABC transporters: a new approach to toxicogenomics....Pages 109-114 SXR and the xenobiotic response....Pages 115-125 Front Matter....Pages 127-127 C. elegans as a tool for environmental toxicology....Pages 129-134 Development of the test method for detection of endocrine-disrupting activity using DNA microarrays....Pages 135-140 Application of cDNA microarray for uterotrophic assay....Pages 141-148 Large-scale gene expression analysis for evaluation of endocrine disruptors....Pages 149-155 Analysis of toxicogenomic response to endocrine disruptors in the mouse testis....Pages 156-162 Front Matter....Pages 127-127 Gene expression profiling to identify mechanisms of male reproductive toxicity....Pages 163-168 Front Matter....Pages 169-169 Toxicology and Genetic Toxicology in the New Era of “Toxicogenomics”: Impact of “-omics” Technologies....Pages 171-193 Use of Genomics in Mechanism Based Risk Assessment....Pages 194-203 Chemogenomics for Predictive Drug Assessment....Pages 204-212 Reverse toxicology as a future predictive toxicology....Pages 213-218 Back Matter....Pages 219-228