Tumor Organoids

Description

Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heterogeneity of in vivo tissue, and are suitable for tumor bioengineering research. Microengineering technologies have resulted in advanced methods for creating and culturing 3-D human tissue. By encapsulating the respective cell type or combining several cell types to form tissues, these model organs can be viable for longer periods of time and are cultured to develop functional properties similar to native tissues. This approach recapitulates the dynamic role of cell–cell, cell–ECM, and mechanical interactions inside the tumor. Further incorporation of cells representative of the tumor stroma, such as endothelial cells (EC) and tumor fibroblasts, can mimic the in vivo tumor microenvironment. Collectively, bioengineered tumors create an important resource for the in vitro study of tumor growth in 3D including tumor biomechanics and the effects of anti-cancer drugs on 3D tumor tissue. These technologies have the potential to overcome current limitations to genetic and histological tumor classification and development of personalized therapies. Front Matter ....Pages i-xvi Techniques to Produce and Culture Lung Tumor Organoids (Cameron Yamanishi, Kimberly Jen, Shuichi Takayama)....Pages 1-15 Tissue Organoids: Liver (Estela Solanas, Iris Pla-Palacín, Pilar Sainz-Arnal, Manuel Almeida, Alberto Lue, Trinidad Serrano et al.)....Pages 17-33 Mammary Gland Organoids (Rocío Sampayo, Sol Recouvreux, María Inés Diaz Bessone, Marina Simian)....Pages 35-49 Biofabrication Technologies for Developing In Vitro Tumor Models (Andrea Mazzocchi, Shay Soker, Aleksander Skardal)....Pages 51-70 Three Dimensional In Vitro Tumor Platforms for Cancer Discovery (Manasa Gadde, Dan Marrinan, Rhys J. Michna, Marissa Nichole Rylander)....Pages 71-94 Tissue-Engineered Models for Studies of Bone Metastasis (Aaron E. Chiou, Claudia Fischbach)....Pages 95-116 Building Better Tumor Models: Organoid Systems to Investigate Angiogenesis (Venktesh S. Shirure, Mary Kathryn Sewell-Loftin, Sandra F. Lam, Tyson D. Todd, Priscilla Y. Hwang, Steven C. George)....Pages 117-148 Microfluidics in Cell and Tissue Studies (Shiny Amala Priya Rajan, Parker Hambright, Rosemary Clare Burke, Adam R. Hall)....Pages 149-170 Stiffness-Tuned Matrices for Tumor Cell Studies (Amanda M. Smelser, Manuel M. Gomez, Scott Smyre, Melissa L. Fender Pashayan, Jed C. Macosko)....Pages 171-191 Mathematical Modeling of Tumor Organoids: Toward Personalized Medicine (Aleksandra Karolak, Katarzyna A. Rejniak)....Pages 193-213