Preeclampsia : Methods and Protocols

Description

This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques. Cutting-edge and comprehensive, Pre-Eclampsia: Methods and Protocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship. Front Matter ....Pages i-xv Diagnostic Imaging: Ultrasound (Stefan C. Kane, Su Lynn Khong, Fabricio da Silva Costa)....Pages 1-8 Biomarker Immunoassays in the Diagnosis of Preeclampsia: Calculating the sFlt1/PlGF Ratio Using the Cobas®e 411 Analyser (Carin Black, Fabricio da Silva Costa)....Pages 9-26 Assessing the Circulating Placental-Specific Anti-angiogenic Protein sFLT-1 e15a in Preeclampsia (Kirsten Palmer)....Pages 27-37 Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Preeclampsia (Sebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, Euan M. Wallace)....Pages 39-52 Genetic Approaches in Preeclampsia (Hannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, Eric K. Moses)....Pages 53-72 Epigenetics and Preeclampsia: Programming of Future Outcomes (Alberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, Edward Araujo Júnior)....Pages 73-83 Inflammatory and Immune System Markers (Kelly J. McKelvey, Gaayathri Ariyakumar, Sharon A. McCracken)....Pages 85-101 Methods to Enrich Exosomes from Conditioned Media and Biological Fluids (Shayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, Carlos Salomon)....Pages 103-115 Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants (Mancy Tong, Lawrence W. Chamley)....Pages 117-129 Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation (Andrew Lai, Omar Elfeky, Gregory E. Rice, Carlos Salomon)....Pages 131-138 Proteomics Method to Identification of Protein Profiles in Exosomes (Andrew Lai, Vyjayanthi Kinhal, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, Carlos Salomon)....Pages 139-153 Harvesting and Characterization of Syncytial Nuclear Aggregates Following Culture of First Trimester Human Placental Explants (Priyadarshini Pantham, Lawrence W. Chamley)....Pages 155-163 Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index (Severine A. Degrelle, Thierry Fournier)....Pages 165-171 Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of Off-Target Effects (Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, Padma Murthi)....Pages 173-189 Immunohistological Techniques (Evangelina Capobianco, Nora Martinez)....Pages 191-201 Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin (Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, Carlos Salomon)....Pages 203-217 Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta (Hélène Clabault, Laetitia Laurent, J. Thomas Sanderson, Cathy Vaillancourt)....Pages 219-231 Analyzing Trophoblast Function Using Cell-Based Assays (Katie L. Powell, Anthony W. Ashton)....Pages 233-246 Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis (Gina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, Bill Kalionis)....Pages 247-266 An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro (Tejasvy Chollangi, Hélène Clabault, Andrée-Anne Hudon Thibeault, Hannah E. J. Yong, Shagun Narula, Ellen Menkhorst et al.)....Pages 267-276 In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases (Lucas Sagrillo-Fagundes, Laetitia Laurent, Josianne Bienvenue-Pariseault, Cathy Vaillancourt)....Pages 277-283 Measurement of Oxidative Stress: Mitochondrial Function Using the Seahorse System (Dilys T. H. Leung, Simon Chu)....Pages 285-293 Co-culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-placental Interactions: Focus on Estrogen Biosynthesis (Andrée-Anne Hudon Thibeault, J. Thomas Sanderson, Cathy Vaillancourt)....Pages 295-304 Placental Lipid Transport (Evemie Dubé, Guillaume Desparois, Julie Lafond)....Pages 305-316 EG-VEGF Maintenance Over Early Gestation to Develop a Pregnancy-Induced Hypertensive Animal Model (Déborah Reynaud, Frédéric Sergent, Roland Abi Nahed, Sophie Brouillet, Mohamed Benharouga, Nadia Alfaidy)....Pages 317-324 Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Preeclampsia (Bryan Leaw, Seshini Gurusinghe, Rebecca Lim, Euan M. Wallace)....Pages 325-334 Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Preeclampsia (Sebastian R. Hobson, Rebecca Lim, Euan M. Wallace)....Pages 335-345 A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Preeclampsia in Moderate- and High-Risk Women: The MELPOP Trial (Sebastian R. Hobson, Euan M. Wallace, John C. Kingdom, Ryan J. Hodges)....Pages 347-352 Back Matter ....Pages 353-355