Photosynthesis

Description

This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Survey of Tools for Measuring In Vivo Photosynthesis (Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, Tracy Lawson)....Pages 3-24 Photosynthetic Gas Exchange in Land Plants at the Leaf Level (Florian A. Busch)....Pages 25-44 Design and Use of a Digitally Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants (Gavin M. George, Katharina Kölling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flütsch, Samuel C. Zeeman)....Pages 45-68 Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS) (Qingfeng Song, Xin-Guang Zhu)....Pages 69-81 Light-Response Curves in Land Plants (Robert A. Coe, HsiangChun Lin)....Pages 83-94 Chlorophyll Fluorescence on the Fast Timescale (Olubukola O. Ajigboye, Rumiana V. Ray, Erik H. Murchie)....Pages 95-104 Sub-saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield (Thomas J. Avenson, Aaron J. Saathoff)....Pages 105-120 Chlorophyll Fluorescence Imaging (Tracy Lawson, Silvere Vialet-Chabrand)....Pages 121-140 Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry (Steven M. Driever, Neil R. Baker)....Pages 141-154 Using Stable Carbon Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations (Nerea Ubierna, Meisha-Marika Holloway-Phillips, Graham D. Farquhar)....Pages 155-196 Liquid-Phase Measurements of Photosynthetic Oxygen Evolution (Dmitriy Shevela, Wolfgang P. Schröder, Johannes Messinger)....Pages 197-211 Front Matter ....Pages 213-213 Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting (J. Alejandro Perdomo, Cristina R. G. Sales, Elizabete Carmo-Silva)....Pages 215-227 Extraction of RuBisCO to Determine Catalytic Constants (Douglas J. Orr, Elizabete Carmo-Silva)....Pages 229-238 Spectrophotometric Determination of RuBisCO Activity and Activation State in Leaf Extracts (Cristina R. G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, Elizabete Carmo-Silva)....Pages 239-250 Front Matter ....Pages 251-251 Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features (Roxana Khoshravesh, Tammy L. Sage)....Pages 253-261 Determining the Subcellular Localization of Fluorescently Tagged Proteins Using Protoplasts Extracted from Transiently Transformed Nicotiana benthamiana Leaves (Vivien Rolland)....Pages 263-283 3D Clearing and Molecular Labeling in Plant Tissues (William M. Palmer, Jamie R. Flynn, Antony P. Martin, Stephanie L. Reed, Christopher P. L. Grof, Rosemary G. White et al.)....Pages 285-304 Evaluation of Lipids for the Study of Photosynthetic Membranes (Helmut Kirchhoff, Robert Yarbrough)....Pages 305-316 Front Matter ....Pages 317-317 “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs (Jackson D. Megiatto Jr., Catia Ornelas)....Pages 319-334 Biophotovoltaics: Design and Study of Bioelectrochemical Systems for Biotechnological Applications and Metabolic Investigation (Stephen J. L. Rowden, Paolo Bombelli, Christopher J. Howe)....Pages 335-346 Back Matter ....Pages 347-355