Plant Meiosis: Methods and Protocols

Description

This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. Front Matter ....Pages i-xiv Front Matter ....Pages 1-1 A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis (Maria Ada Prusicki, Yuki Hamamura, Arp Schnittger)....Pages 3-12 Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes (Santiago Valentín Galvan Gordillo, Rocio Escobar-Guzman, Daniel Rodriguez-Leal, Jean-Philippe Vielle-Calzada, Arnaud Ronceret)....Pages 13-24 How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana (Pablo Parra-Nunez, Mónica Pradillo, Juan Luis Santos)....Pages 25-36 Cytological Characterization of Arabidopsis arenosa Polyploids by SIM (Chris Morgan, Eva Wegel)....Pages 37-46 Targeted Analysis of Chromatin Events (TACE) (Jason Sims, Changbin Chen, Peter Schlögelhofer, Marie-Therese Kurzbauer)....Pages 47-58 Whole-Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) (Jason Sims, Jihed Chouaref, Peter Schlögelhofer)....Pages 59-66 Front Matter ....Pages 67-67 Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea (Zeeshan Shamim, Susan J. Armstrong)....Pages 69-78 Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization (Stephen M. Stack, Lindsay A. Shearer, Leslie D. Lohmiller, Lorinda K. Anderson)....Pages 79-115 Cytological Techniques to Study Cytomixis in Plant Male Meiosis (Sergey R. Mursalimov, Yuriy V. Sidorchuk, Elena V. Deineko)....Pages 117-129 Analysis of Meiosis in Nonmodel Tropical Plants: The Case of Carica papaya Linn (José Mora-Calderón, Kalani Scott-Moraga, Pablo Bolaños-Villegas)....Pages 131-139 Analytical Methodology of Meiosis in Autopolyploid and Allopolyploid Plants (Pilar Prieto, Tomás Naranjo)....Pages 141-168 Multicolored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids (Virginie Huteau, Olivier Coriton)....Pages 169-180 Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy (Nieves Cuñado)....Pages 181-196 Quantification of Synapsis Using Immunolocalization in Embedded Nuclei of Lolium (Dylan W. Phillips)....Pages 197-206 Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy (Benoit Darrier, Mikel Arrieta, Sybille U. Mittmann, Pierre Sourdille, Luke Ramsay, Robbie Waugh et al.)....Pages 207-215 Front Matter ....Pages 217-217 Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers (Christophe Lambing, Kyuha Choi, Alexander R. Blackwell, Ian R. Henderson)....Pages 219-236 Isolating Male Meiocytes from Maize and Wheat for “-Omics” Analyses (Stefanie Dukowic-Schulze, Nelson Garcia, Arun S. K. Shunmugam, Sateesh Kagale, Changbin Chen)....Pages 237-258 How to Study the Proteomes and Phosphoproteomes of Anther and Pollen (Chi Zhang, Xiaobing Feng, Menghui Hu, Zaibao Zhang)....Pages 259-265 Rice Female Meiosis: Genome-Wide mRNA, Small RNA, and DNA Methylation Analysis During Ovule Development (Helian Liu, Aqin Cao, Liyu Yang, Jianbo Wang)....Pages 267-280 Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei (Steven Dreissig, Jörg Fuchs, Axel Himmelbach, Martin Mascher, Andreas Houben)....Pages 281-300 Front Matter ....Pages 301-301 Identifying and Isolating Meiotic Mutants in a Polyploid Brassica Crop (Marina Pfalz, Adrián Gonzalo, Nicolas Christophorou, Aurélien Blary, Aurélie Berard, Nadia Bessoltane et al.)....Pages 303-318 A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS) (Stuart Desjardins, Kostya Kanyuka, James D. Higgins)....Pages 319-330 Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana (Nico De Storme, Danny Geelen)....Pages 331-346 Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species (Dan D. Wu, Alevtina Ruban, Twan Rutten, Yong H. Zhou, Andreas Houben)....Pages 347-358 Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants (Annika Dorn, Holger Puchta)....Pages 359-366 A Modular Tray Growth System for Barley (Mikel Arrieta, Isabelle Colas, Malcolm Macaulay, Robbie Waugh, Luke Ramsay)....Pages 367-379 In Planta Delivery of Chemical Compounds into Barley Meiocytes: EdU as Compound Example (Yun-Jae Ahn, Maria Cuacos, Mohammad A. Ayoub, Judith Kappermann, Andreas Houben, Stefan Heckmann)....Pages 381-402 Back Matter ....Pages 403-405