Plant Chemical Genomics

Description

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches. Front Matter ....Pages i-xi Multi-Parametric Screening in Arabidopsis thaliana Seedlings (Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, Dominique Audenaert)....Pages 1-7 Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress (Susan Fozard, Brian G. Forde)....Pages 9-25 Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen (Roman Chuprov-Netochin, Elena Marusich, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Yan Ivanenkov et al.)....Pages 27-37 A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death Using Suspension Cell Culture of Arabidopsis thaliana (Yoshiteru Noutoshi, Ken Shirasu)....Pages 39-47 High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression (Vivek Halder, Erich Kombrink)....Pages 49-63 Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics (Clémence Bonnot, Laurent Nussaume, Thierry Desnos)....Pages 65-84 Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls Using BY-2 Cells (Emiko Okubo-Kurihara, Minami Matsui)....Pages 85-92 Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes (Mitsuhiro Kimura, Sachiko Isobe)....Pages 93-99 High-Throughput In Vitro Screening for Inhibitors of Cereal α-Glucosidase (Michael D. Rugen, Martin Rejzek, Henrik Naested, Birte Svensson, Robert A. Field)....Pages 101-115 Chemical Screening for Strigolactone Receptor Antagonists Using Arabidopsis thaliana (Duncan Holbrook-Smith, Peter McCourt)....Pages 117-126 Chemical Control of ABA Receptors to Enable Plant Protection Against Water Stress (Masanori Okamoto, Sean R. Cutler)....Pages 127-141 Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening (Yajin Ye, Yang Zhao)....Pages 143-148 Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside That Controls the Leaf Closure of Samanea saman (Yousuke Takaoka, Minoru Ueda)....Pages 149-158 Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule (Yoichi Takakusagi, Kaori Takakusagi, Fumio Sugawara, Kengo Sakaguchi)....Pages 159-172 Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants Using PlantiSMASH (Satria A. Kautsar, Hernando G. Suarez Duran, Marnix H. Medema)....Pages 173-188 Chemical Genomics Translatability from Unicellular to Multicellular Models (Cecilia Rodriguez-Furlán, Carlos Rubilar-Hernández, Lorena Norambuena)....Pages 189-201 Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii (Brittany Jack, Prachee Avasthi)....Pages 203-221 FnCpf1-Mediated Targeted Mutagenesis in Plants (Akira Endo, Seiichi Toki)....Pages 223-239 Erratum to: Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii (Brittany Jack, Prachee Avasthi)....Pages E1-E1 Back Matter ....Pages 241-242