Cell Culture Techniques

Description

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field. Front Matter ....Pages i-xiii In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model (Emma H. Neal, Yajuan Shi, Ethan S. Lippmann)....Pages 1-15 In Vitro Techniques for Assessing Neurotoxicity Using Human iPSC-Derived Neuronal Models (Anke M. Tukker, Fiona M. J. Wijnolts, Aart de Groot, Richard W. Wubbolts, Remco H. S. Westerink)....Pages 17-35 Oxidative Stress Signatures in Human Stem Cell-Derived Neurons (M. Diana Neely, Aaron B. Bowman)....Pages 37-49 Glial Reactivity in Response to Neurotoxins: Relevance and Methods (Lindsay T. Michalovicz, James P. O’Callaghan)....Pages 51-67 Neuron-Glia Interactions Studied with In Vitro Co-Cultures (S. Mancino, M. M. Serafini, Barbara Viviani)....Pages 69-85 Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria (Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, Jeremy W. Chambers)....Pages 87-118 Epigenetic Changes in Cultures: Neurons and Astrocytes (David P. Gavin, Xiaolu Zhang, Marina Guizzetti)....Pages 119-139 The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation (Laura Nimtz, Jördis Klose, Stefan Masjosthusmann, Marta Barenys, Ellen Fritsche)....Pages 141-168 Zebrafish as a Tool to Assess Developmental Neurotoxicity (Keturah G. Kiper, Jennifer L. Freeman)....Pages 169-193 Rat Brain Slices: An Optimum Biological Preparation for Acute Neurotoxicological Studies (Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya-López, Edgar Rangel-López, Abel Santamaría)....Pages 195-207 Electrophysiological Neuromethodologies (Yukun Yuan, William D. Atchison)....Pages 209-231 A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies (Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller et al.)....Pages 233-243 Transporter Studies: Brain Punching Technique (Cherish A. Taylor, Somshuvra Mukhopadhyay)....Pages 245-253 miRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders (Lena Smirnova, Alexandra Maertens)....Pages 255-281 Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes (Anna Bal-Price, Francesca Pistollato)....Pages 283-299 Back Matter ....Pages 301-302