ENGLISH

Circulating MicroRNAs: Methods and Protocols

By Nobuyoshi Kosaka , Yusuke Yoshioka , Keitaro Hagiwara (auth.) , Nobuyoshi Kosaka (eds.)

Book information

Publisher
Humana Press
Year
2013
ISBN
1627034528, 9781627034524
DOI
10.1007/978-1-62703-453-1
ISSN
1064-3745
LCC
2013939032
Language
english
Format
PDF
Filesize
4 MB (4233303 bytes)
Series
Methods in Molecular Biology 1024
Edition
1
Pages
270\283
Topic
Biology Genetics
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2013-08-04 03:54:17
Human Genetics

Description

microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.

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