Blood-Brain Barrier

Description

This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches. Front Matter ....Pages i-xiii An Overview of the Blood-Brain Barrier (Tatiana Barichello, Allan Collodel, Rodrigo Hasbun, Rodrigo Morales)....Pages 1-8 Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics (Brian M. Kopec, Kavisha R. Ulapane, Mario E. G. Moral, Teruna J. Siahaan)....Pages 9-43 Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modeling of the Blood-Brain Barrier (Malgorzata Burek, Carola Y. Förster)....Pages 45-54 In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems (Mohammad A. Kaisar, Vinay V. Abhyankar, Luca Cucullo)....Pages 55-70 In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress on Brain Microvascular Endothelial Cell Phenotype (Keith D. Rochfort, Philip M. Cummins)....Pages 71-98 Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier (Balaji Srinivasan, Aditya Reddy Kolli)....Pages 99-114 Cell-Penetrating Peptides as Theranostics Against Impaired Blood-Brain Barrier Permeability: Implications for Pathogenesis and Therapeutic Treatment of Neurodegenerative Disease (Swapna Bera, Anirban Bhunia)....Pages 115-136 Microbial Translocation of the Blood-Brain Barrier (Charles T. Spencer, Mireya G. Ramos Muniz)....Pages 137-161 Transport Across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System (Sen Takeda, Keishi Narita)....Pages 163-173 Drosophila as a Model to Study the Blood-Brain Barrier (Cameron R. Love, Brigitte Dauwalder)....Pages 175-185 Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier (Tianzhi Yang, Shuhua Bai)....Pages 187-196 Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents (Fabricia Petronilho, Julia L. Goldman, Tatiana Barichello)....Pages 197-203 Experimental Tools to Study the Regulation and Function of the Choroid Plexus (Isabel Gonçalves, Telma Quintela, Ana Catarina Duarte, Peter Hubbard, Graça Baltazar, Christian Schwerk et al.)....Pages 205-230 Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid Across the Blood-Cerebrospinal Fluid Barrier (Shin-ichi Akanuma, Yoshiyuki Kubo, Ken-ichi Hosoya)....Pages 231-248 In Vivo Analysis to Study Transport Across the Blood-Retinal Barrier (Yoshiyuki Kubo, Shin-ichi Akanuma, Ken-ichi Hosoya)....Pages 249-265 Increasing BBB Permeability via Focused Ultrasound: Current Methods in Preclinical Research (Dallan McMahon, Charissa Poon, Kullervo Hynynen)....Pages 267-297 Evaluation of Blood–Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evans Blue Extravasation (Trish Domi, Faraz Honarvar, Andrea Kassner)....Pages 299-314 Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy (Aline Silva de Miranda, Thiago Macedo Cordeiro, Milene Alvarenga Rachid, Antônio Lúcio Teixeira)....Pages 315-327 Molecular Imaging of Blood–Brain Barrier Permeability in Preclinical Models Using PET and SPECT (Vijayasree V. Giridharan, Tatiana Barichello, Sudhakar Selvaraj)....Pages 329-342 Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement (Pavani Sayana, Jean Pierre Oses, Tatiana Barichello, Vijayasree V. Giridharan)....Pages 343-363 Back Matter ....Pages 365-367