ENGLISH

Pyrosequencing® Protocols

By John M. Walker , Pål Nyrén (auth.) , Sharon Marsh (eds.)

Book information

Publisher
Humana Press
Year
2007
ISBN
1588296458, 9781588296450
DOI
10.1385/1597453773
Open Library ID
OL17208766M
Language
english
Format
PDF
Filesize
2 MB (1823383 bytes)
Series
Methods in Molecular Biology 373
Edition
1
Pages
196\203
Scanned
yes
Time added
2012-02-04 16:00:00
Human Genetics

Description

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

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