Macrophages

Description

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level. The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com. Front Matter ....Pages i-xii Culture of Human Monocyte-Derived Macrophages (Aoife Kelly, Aleksander M. Grabiec, Mark A. Travis)....Pages 1-11 A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages (Chandrayana Mukherjee, Christine Hale, Subhankar Mukhopadhyay)....Pages 13-28 Growing Murine Bone Marrow-Derived Macrophages (Anaïs Assouvie, Lisa P. Daley-Bauer, Germain Rousselet)....Pages 29-33 Immortalization of Murine Bone Marrow-Derived Macrophages (Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz)....Pages 35-49 Isolation and Identification of Murine Serous Cavity Macrophages (Calum C. Bain, Stephen J. Jenkins)....Pages 51-67 Isolation and Identification of Interstitial Macrophages from the Lungs Using Different Digestion Enzymes and Staining Strategies (Shaikh M. Atif, Sophie L. Gibbings, Claudia V. Jakubzick)....Pages 69-76 Isolation and Phenotyping of Adult Mouse Microglial Cells (Kathleen Grabert, Barry W. McColl)....Pages 77-86 Isolation and Phenotyping of Bone Marrow Macrophages (Marion Chalot)....Pages 87-92 Isolation and Phenotyping of Intestinal Macrophages (Vanessa Petit)....Pages 93-98 Phenotyping Tumor-Associated Macrophages (R. Ian Cumming, Yen-Rei A. Yu)....Pages 99-109 Activating Murine Macrophages In Vitro (Elizabeth Dalby)....Pages 111-117 Polarizing Macrophages In Vitro (Xuan Huang, Yong Li, Mingui Fu, Hong-Bo Xin)....Pages 119-126 Viral Replication Assay in Bone Marrow-Derived Macrophages (Linda Roback, Lisa P. Daley-Bauer)....Pages 127-134 Macrophage Bactericidal Assays (Mourad Aribi)....Pages 135-149 Quantitative Phagocytosis Assays in Primary and Cultured Macrophages (Fernando Montaño, Sergio Grinstein, Roni Levin)....Pages 151-163 Observing Frustrated Phagocytosis and Phagosome Formation and Closure Using Total Internal Reflection Fluorescence Microscopy (TIRFM) (Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, Florence Niedergang)....Pages 165-175 Chromatin Immunoprecipitation in Macrophages (Germain Rousselet)....Pages 177-186 Transfecting Macrophages (Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Scheiding, Stefan Lorkowski)....Pages 187-195 In Vitro Migration Assays (Lewis Taylor, Carlota Recio, David R. Greaves, Asif J. Iqbal)....Pages 197-214 Cytokine-Induced Acute Inflammatory Monoarticular Arthritis (Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, Andrew D. Cook)....Pages 215-223 Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections (Dominik Rückerl)....Pages 225-241 Genetic Models of Macrophage Depletion (Li Hua, Jiayuan Shi, Leonard D. Shultz, Guangwen Ren)....Pages 243-258 Depleting Macrophages In Vivo with Clodronate-Liposomes (Stéphanie G. Moreno)....Pages 259-262 Cre Driver Mice Targeting Macrophages (Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, Guangwen Ren)....Pages 263-275 Back Matter ....Pages 277-278