ENGLISH

Detection of Blotted Proteins: Methods and Protocols

By Biji T. Kurien , R. Hal Scofield

Book information

Publisher
Humana Press
Year
2015
ISBN
1493927175, 9781493927173
DOI
10.1007/978-1-4939-2718-0
ISSN
1064-3745
LCC
2015941322
Language
english
Format
PDF
Filesize
12 MB (12385099 bytes)
Series
Methods in Molecular Biology 1314
Pages
xiv+386\401
Topic
Biology Molecular
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-07-08 11:24:45
Protein Science

Description

This detailed volume provides methods and techniques for detection after blotting.  Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

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