Amyloid Proteins: Methods and Protocols

Description

Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word “amyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analyticalmethods for studying these proteins, cell culture models andassays for production of amyloid proteins, and protocols foramyloid extraction from tissue, its detection in vitro and in vivo, as well asnontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories. Front Matter....Pages i-xv Front Matter....Pages 1-1 Front Matter....Pages 3-10 Front Matter....Pages 11-21 Front Matter....Pages 23-31 Back Matter....Pages 33-52 ....Pages 53-68