Type 2 Diabetes: Methods and Protocols

Description

This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field. Front Matter ....Pages i-x Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now? (John C. Clapham)....Pages 1-30 Practical Considerations for In Vivo Mouse Studies (Edward T. Wargent)....Pages 31-42 Nutritional Models of Type 2 Diabetes Mellitus (Beverly Sara Mühlhäusler, Carla Toop, Sheridan Gentili)....Pages 43-69 Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes (Paul W. Finn)....Pages 71-84 Whole-Exome Sequencing (WES) for Illumina Short Read Sequencers Using Solution-Based Capture (Milind C. Mahajan, Andrew S. McLellan)....Pages 85-108 Gene Expression Mining in Type 2 Diabetes Research (Donald R. Dunbar)....Pages 109-117 Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes (Maysson Ibrahim)....Pages 119-128 Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia (Jayne A. L. Houghton)....Pages 129-177 Isolation and Purification of Rodent Pancreatic Islets of Langerhans (Jacqueline F. O’Dowd, Claire J. Stocker)....Pages 179-184 Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry (Matthew J. Butcher, Michelle B. Trevino, Yumi Imai, Elena V. Galkina)....Pages 185-197 Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation (Ionel Sandovici, Lisa M. Nicholas, Laura P. O’Neill)....Pages 199-213 Quantification of Pancreatic Islets: Using Image Analysis Tools (Parvathy E. Harikumar)....Pages 215-229 Measurement of Calcium Signaling in Beta-Cell Lines Using Epifluorescence and Confocal Microscopy (Joanne L. Selway)....Pages 231-239 Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells (Rodrigo Carlessi, Vinicius Cruzat, Younan Chen, Philip Newsholme)....Pages 241-253 Primary Adipocytes as a Model for Insulin Sensitivity (Mohamed S. Zaibi)....Pages 255-263 Assessing Islet Transplantation Outcome in Mice (Aileen J. F. King, Chloe L. Rackham)....Pages 265-280 Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent (Edward T. Wargent)....Pages 281-297 Back Matter ....Pages 299-300