Rheumatoid Arthritis: Methods and Protocols

Description

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study. Front Matter ....Pages i-ix Front Matter ....Pages 1-1 Collagen-Induced Arthritis Models (Maya Miyoshi, Shuang Liu)....Pages 3-7 Human Xenograft Model (Shuang Liu)....Pages 9-15 Long-Term Constant Subcutaneous Drug Administration (Shuang Liu, Maya Miyoshi)....Pages 17-22 Clinical Scoring of Disease Activity in Animal Models (Maya Miyoshi, Shuang Liu)....Pages 23-28 Histological Analysis of Arthritic Joints (Takeshi Kiyoi)....Pages 29-39 Preparation of Joint Extracts (Shuang Liu, Erika Takemasa)....Pages 41-45 Front Matter ....Pages 47-47 Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System (Wei Zhou, Hiroyuki Takeda)....Pages 49-67 Reconstruction of Protein/Liposome Complex (Yasuyuki Suzuki)....Pages 69-77 Production of Neutralizing Antibody (Erika Takemasa, Shuang Liu, Hitoshi Hasegawa)....Pages 79-92 Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen (Hiroyuki Takeda)....Pages 93-112 Generation of Specific Aptamers (Shuang Liu, Yasuyuki Suzuki, Makoto Inui)....Pages 113-121 Production of Lentiviral Particles (Shuang Liu)....Pages 123-128 RNA Interference Ex Vivo (Shuang Liu)....Pages 129-135 Lentiviral-Mediated Systemic RNA Interference In Vivo (Shuang Liu)....Pages 137-144 Mesenchymal Stem Cell Engineering (Shuang Liu)....Pages 145-150 Front Matter ....Pages 151-151 Screening of Ca2+ Influx in Lymphocytes (Erika Takemasa, Shuang Liu)....Pages 153-159 Single-Cell Ca2+ Imaging (Shuang Liu)....Pages 161-168 Electrophysiological Methods to Measure Ca2+ Current (Shuang Liu, Miyuki Kuno)....Pages 169-176 The Functional Assessment of T cells (Saho Maruyama)....Pages 177-199 Release of Antibodies and Cytokines from B Cells (Shuang Liu)....Pages 201-207 Evaluation of Autoreactive Responses (Shuang Liu)....Pages 209-213 Bone Resorption Activity in Mature Osteoclasts (Takeshi Kiyoi)....Pages 215-222 Animal Models of Vasculitis (M. Mogi, Shuang Liu)....Pages 223-232 Front Matter ....Pages 233-233 Design an Intervention Study (Hitoshi Hasegawa)....Pages 235-241 Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis (Hitoshi Hasegawa)....Pages 243-250 Correction to : Production of Lentiviral Particles (Shuang Liu)....Pages E1-E1 Back Matter ....Pages 251-253