Laboratory Experiments in Microbiology

Description

Containing 57 thoroughly class-tested and easily customizable exercises, Laboratory Experiments in Microbiology, Tenth Edition, provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction or any introductory microbiology text, the Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as questions relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique. Cover Table of Contents 1. Introduction 2. Microscopy 3. Use and Care of the Microscope 4. Examination of Living Microorganisms 5. Handling Bacteria 6. Microbes in the Environment 7. Transfer of Bacteria: Aseptic Technique 8. Staining Methods 9. Preparation of Smears and Simple Staining 10. Gram Staining 11. Negative Staining 12. Acid-Fast Staining 13. Structural Stains (Endospore, Capsule, and Flagella) 14. Morphologic Unknown 15. Cultivation of Bacteria 16. Isolation of Bacteria by Dilution Techniques 17. Special Media for Isolating Bacteria 18. Microbial Metabolism 19. Carbohydrate Catabolism 20. Fermentation 21. Protein Catabolism, Part 1 22. Protein Catabolism, Part 2 23. Respiration 24. Unknown Identification and Bergey's Manual 25. Microbial Growth 26. Oxygen and the Growth of Bacteria 27. Determination of a Bacterial Growth Curve: The Role of Temperature 28. Biofilms 29. Control of Microbial Growth 30. Physical Methods of Control: Ultraviolet Radiation 31. Physical Methods of Control: Heat 32. Chemical Methods of Control: Antimicrobial Drugs 33. Chemical Methods of Control: Disinfectants and Antiseptics 34. Effectiveness of Hand Scrubbing 35. Microbial Genetics 36. Regulation of Gene Expression 37. Isolation of Bacterial Mutants 38. Transformation of Bacteria 39. DNA Fingerprinting 40. Genetic Engineering 41. Ames Test for Detecting Possible Chemical Carcinogens 42. The Microbial World 43. Fungi: Yeasts and Molds 44. Phototrophs: Algae and Cyanobacteria 45. Protozoa 46. Parasitic Helminths 47. Viruses 48. Isolation and Titration of Bacteriophages 49. Plant Viruses 50. Interaction of Microbe and Host 51. Epidemiology 52. Koch's Postulates 53. Immunology 54. Innate Immunity 55. Agglutination Reactions: Slide Agglutination 56. Agglutination Reactions: Microtiter Agglutination 57. ELISA Technique 58. Microorganisms and Disease 59. Bacteria of the Skin 60. Bacteria of the Respiratory Tract 61. Bacteria of the Mouth 62. Bacteria of the Gastrointestinal Tract 63. Bacteria of the Genitourinary Tract 64. Identification of an Unknown from a Clinical Sample 65. Rapid Identification Methods 66. Microbiology and the Environment 67. Microbes in Water: Multiple-Tube Technique 68. Microbes in Water: Membrane Filter Technqiue 69. Microbes in Food: Contamination 70. Microbes Used in the Production of Foods 71. Microbes in Soil: The Nitrogen and Sulfur Cycles 72. Microbes in Soil: Bioremediation 73. Appendix: Pipetting 74. Appendix: Dilution Techniques and Calculations 75. Appendix: Use of the Spectrophotometer 76. Appendix: Graphing 77. Appendix: Use of the Dissecting Microscope 78. Appendix: Use of the Membrane Filter 79. Appendix: Electrophoresis 80. Appendix: Keys to Bacteria Index A B C D E F G H I J K L M N O P Q R S T U V W X Y Z