Laboratory Experiments in Microbiology (What's New in Microbiology)

Description

For general microbiology laboratory courses Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction, 13th Edition or any introductory microbiology text.   The 12th Edition of Laboratory Experiments in Microbiology is easier than ever to navigate and more visually effective with new icons indicating when an exercise addresses the human or environmental microbiome, is investigative, or addresses an ASM guideline. New ASM Thinking Skills outline the steps that help develop laboratory thinking skills. Pre-lab quizzes in Mastering™ Microbiology ensure students arrive prepared for each lab, and activities such as Lab and Lecture: Putting It All Together help students see how lab and lecture are integrated.   Front Cover General Safety in the Laboratory Title Page Copyright Page Contents Preface Introduction Student Safety Contract Part 1 Microscopy 1 Use and Care of the Microscope 2 Examination of Living Microorganisms Part 2 Handling Bacteria 3 Microbes in the Environment 4 Transfer of Bacteria: Aseptic Technique Part 3 Staining Methods 5 Preparation of Smears and Simple Staining 6 Negative Staining 7 Gram Staining 8 Acid-Fast Staining 9 Structural Stains (Endospore, Capsule, and Flagella) 10 Morphologic Unknown Part 4 Cultivation of Bacteria 11 Isolation of Bacteria by Dilution Techniques 12 Special Media for Isolating Bacteria Part 5 Microbial Metabolism 13 Carbohydrate Catabolism 14 Fermentation 15 Protein Catabolism, Part 1 16 Protein Catabolism, Part 2 17 Respiration 18 Unknown Identification and Bergey’s Manual Part 6 Microbial Growth 19 Oxygen and the Growth of Bacteria 20 Determination of a Bacterial Growth Curve: The Role of Temperature 21 Biofilms Part 7 Control of Microbial Growth 22 Physical Methods of Control: Heat 23 Physical Methods of Control: Ultraviolet Radiation 24 Chemical Methods of Control: Disinfectants and Antiseptics 25 Chemical Methods of Control: Antimicrobial Drugs 26 Effectiveness of Hand Scrubbing Part 8 Microbial Genetics 27 Regulation of Gene Expression 28 Isolation of Bacterial Mutants 29 Transformation of Bacteria 30 DNA Fingerprinting 31 Genetic Engineering 32 Ames Test for Detecting Possible Chemical Carcinogens Part 9 The Microbial World 33 Fungi: Yeasts and Molds 34 Phototrophs: Algae and Cyanobacteria 35 Protozoa 36 Parasitic Helminths Part 10 Viruses 37 Isolation and Titration of Bacteriophages 38 Plant Viruses Part 11 Interaction of Microbe and Host 39 Epidemiology 40 Koch’s Postulates Part 12 Immunology 41 Innate Immunity 42 Agglutination Reactions: Slide Agglutination 43 Agglutination Reactions: Microtiter Agglutination 44 ELISA Technique Part 13 Microorganisms and Disease 45 Bacteria of the Skin 46 Bacteria of the Respiratory Tract 47 Bacteria of the Mouth 48 Bacteria of the Gastrointestinal Tract 49 Bacteria of the Genitourinary Tract 50 Identification of an Unknown from a Clinical Sample 51 Rapid Identification Methods Part 14 Microbiology and the Environment 52 Microbes in Water: Multiple-Tube Technique 53 Microbes in Water: Membrane Filter Technique 54 Microbes in Food: Contamination 55 Microbes Used in the Production of Foods 56 Microbes in Soil: The Nitrogen and Sulfur Cycles 57 Microbes in Soil: Bioremediation Appendices A Pipetting B Dilution Techniques and Calculations C Use of the Spectrophotometer D Graphing E Use of the Dissecting Microscope F Use of the Membrane Filter G Electrophoresis H Keys to Bacteria Art and Photography Credits Index Back Cover