Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

Description

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Contents Preface 1. Fundamentals of imaging mass spectrometry 2. Ionization sources for imaging mass spectrometry 3. Sample preparation for imaging mass spectrometry 4. Tissue sectioning for imaging mass spectrometry 5. Matrix for matrix-assisted laser desorption/ionization (MALDI) 6. Molecule identification approaches in imaging mass spectrometry 7. Strategies for quantitative imaging mass spectrometry 8. Spatial resolution of imaging mass spectrometry 9. Visualization in imaging mass spectrometry 10. Data analysis and computation for imaging mass spectrometry 11. Multimodal imaging mass spectrometry 12. Validation and standardization of imaging mass spectrometry 13. Toward clinical imaging mass spectrometry 14. Imaging mass spectrometry: endogenous mammalian metabolites 15. Imaging mass spectrometry: Glycans 16. Imaging mass spectrometry: steroids mapping using on-tissue chemical derivatization 17. Imaging mass spectrometry: neurotransmitter distribution using reactive matrix and chemical derivatization 18. Imaging mass spectrometry: small drugs and metabolites in tissue 19. Imaging mass spectrometry: gangliosides in brain tissue Index