Single Cell Metabolism: Methods and Protocols
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Description
This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. Front Matter ....Pages i-xi Single-Cell Metabolomics by Mass Spectrometry (Bindesh Shrestha)....Pages 1-8 Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry (Linwen Zhang, Linda L. Allworth, Akos Vertes)....Pages 9-18 Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry (Mandy S. Phelps, Guido F. Verbeck)....Pages 19-30 Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis (Zhenwei Wei, Xiaochao Zhang, Xingyu Si, Xiaoyun Gong, Sichun Zhang, Xinrong Zhang)....Pages 31-59 Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells (Ning Pan, Wei Rao, Zhibo Yang)....Pages 61-71 Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics (Alfredo J. Ibáñez, Ales Svatos)....Pages 73-88 Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells (John F. Cahill, Vilmos Kertesz)....Pages 89-101 Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging (Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, Bernhard Spengler)....Pages 103-111 A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population (Jasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, Renato Zenobi)....Pages 113-124 Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry (Bo Yang, Tina Tsui, Richard M. Caprioli, Jeremy L. Norris)....Pages 125-134 Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry (Sylwia A. Stopka, Akos Vertes)....Pages 135-146 Compact Quantum Dots for Quantitative Cytology (Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, Andrew M. Smith)....Pages 147-158 Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos Using Desorption Electrospray Ionization (DESI) Mass Spectrometry (Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, R. Graham Cooks)....Pages 159-179 Spatial Mapping of Cellular Metabolites Using DESI Ion Mobility Mass Spectrometry (Anthony Midey, Hernando Olivos, Bindesh Shrestha)....Pages 181-190 Open-Source Software Tools, Databases, and Resources for Single-Cell and Single-Cell-Type Metabolomics (Biswapriya B. Misra)....Pages 191-217 Ten Major Future Challenges in Single-Cell Metabolomics (Bindesh Shrestha)....Pages 219-223 Back Matter ....Pages 225-226
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