ENGLISH

Nuclear G-Protein Coupled Receptors: Methods and Protocols

By Bruce G. Allen , Terence E. Hébert (eds.)

Book information

Publisher
Humana Press
Year
2015
ISBN
9781493917549, 1493917544
DOI
10.1007/978-1-4939-1755-6
ISSN
1064-3745
LCC
2014949859
Language
english
Format
PDF
Filesize
7 MB (7138528 bytes)
Series
Methods in Molecular Biology 1234
Edition
1
Pages
215\233
Topic
Biology Molecular
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2014-10-26 06:45:42
Protein Science

Description

Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.

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