Epitope Mapping Protocols

Description

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field. Front Matter ....Pages i-xi An Introduction to Epitope Mapping (Johan Nilvebrant, Johan Rockberg)....Pages 1-10 Front Matter ....Pages 11-11 Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography (Moeko Toride King, Cory L. Brooks)....Pages 13-27 Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations (Luca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, Luca Varani)....Pages 29-51 Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors (Diluka Peiris, Teodor Aastrup, Samuel Altun, Camilla Käck, Maria Gianneli, Davide Proverbio et al.)....Pages 53-63 Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically Encoded Photo-Activatable Cross-Linkers (Thomas Huber, Thomas P. Sakmar)....Pages 65-75 Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing (Tariq Ahmad Najar, Shruti Khare, Raghavan Varadarajan)....Pages 77-88 Epitope Mapping Using Yeast Display and Next Generation Sequencing (Thomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton et al.)....Pages 89-118 Front Matter ....Pages 119-119 B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay (Thiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, Michael W. Heuzenroeder)....Pages 121-128 Array-In-Well Epitope Mapping of Phage-Displayed Antibodies (Urpo Lamminmäki, Gaurav Batra, Petri Saviranta)....Pages 129-140 Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries (Anna-Luisa Volk, Francis Jingxin Hu, Johan Rockberg)....Pages 141-157 Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains (Anna-Luisa Volk, Johan Rockberg)....Pages 159-183 Front Matter ....Pages 185-185 Kinetic Analysis and Epitope Binning Using Surface Plasmon Resonance (Johan Nilvebrant)....Pages 187-205 Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry (Lisette Bronswijk-Deddens)....Pages 207-220 Front Matter ....Pages 221-221 Antibody Specificity Profiling Using Protein Microarrays (Pedro Ramos-López, José Irizarry, Ignacio Pino, Seth Blackshaw)....Pages 223-229 High-Density Antigen Microarrays for the Assessment of Antibody Selectivity and Off-Target Binding (Ronald Sjöberg, Eni Andersson, Cecilia Hellström, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson et al.)....Pages 231-238 Multiplexed Antigen Bead Arrays for the Assessment of Antibody Selectivity and Epitope Mapping (Burcu Ayoglu, Peter Nilsson, Jochen M. Schwenk)....Pages 239-248 Antibody Biomarker Discovery Via In Vitro Evolution of Peptides (John T. Ballew, Jack R. Reifert, Patrick S. Daugherty)....Pages 249-268 Bioinformatics Tools for the Prediction of T-Cell Epitopes (Massimo Andreatta, Morten Nielsen)....Pages 269-281 Back Matter ....Pages 283-284