Immunological Tolerance: Methods and Protocols

Description

This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Isolation and Characterisation of Human Adipose-Derived Stem Cells (Anna Wilson, Melisse Chee, Peter Butler, Ashleigh S. Boyd)....Pages 3-13 Isolation of Murine Hematopoietic Stem Cells (Juan Bautista Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng, Neil P. Rodrigues)....Pages 15-23 Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells (Toshinobu Nishimura, Hiromitsu Nakauchi)....Pages 25-40 Front Matter ....Pages 41-41 Isolation of Human Regulatory T Lymphocytes by Fluorescence-Activated Cell Sorting (Kate Milward, Joanna Hester, Kathryn J. Wood)....Pages 43-54 Identification and Isolation of Regulatory B Cells in Mouse and Human (Madhvi Menon, Elizabeth C. Rosser, Claudia Mauri)....Pages 55-66 An Innovative Standard Operation Procedure for Isolating GMP-Grade CD4+CD25+ T Cells from Non-Mobilized Leukapheresis (Wei Zhang, Suzanne M. Watt, David J. Roberts)....Pages 67-83 Front Matter ....Pages 85-85 Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells (Tie Zheng Hou, Omar S. Qureshi, David M. Sansom)....Pages 87-101 Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation In Vitro in the Context of Costimulatory Molecule Blockade (Christine M. Barbon, Kenneth J. Janec, Rowan H. Kelner, James E. Norton, Eva C. Guinan)....Pages 103-118 In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells (Katerina Vlachou, Panayotis Verginis)....Pages 119-128 Disassembling and Reaggregating the Thymus: The Pros and Cons of Current Assays (Elia Piccinini, Paola Bonfanti)....Pages 129-142 Isolation of Highly Viable Thymic Epithelial Cells for Use in In Vitro and In Vivo Experiments (Mi-Jeong Kim, Thomas Serwold)....Pages 143-156 Front Matter ....Pages 157-157 Immune Tolerance and Rejection in Organ Transplantation (Jessica Stolp, Masaaki Zaitsu, Kathryn J. Wood)....Pages 159-180 Method of Generating Tolerogenic Maturation-Resistant Dendritic Cells and Testing for Their Immune-Regulatory Functions In Vivo in the Context of Transplantation (Sherrie J. Divito, Adrian E. Morelli)....Pages 181-193 Front Matter ....Pages 195-195 Luminex-Coupled EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Antigen-Specific T cells in Human Peripheral Blood (HuiJuan Lv, Khadir Raddassi, Myra A. Lipes)....Pages 197-210 Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental Autoimmune Uveitis (Jun Chen, Rachel R. Caspi)....Pages 211-227 Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle (Dalia Burzyn, Amy Wagers, Diane Mathis, Massimiliano Cerletti)....Pages 229-237 Back Matter ....Pages 239-240