ENGLISH

Microbial Gene Techniques

Book information

Publisher
Elsevier, Academic Press
Year
1995
ISBN
0120443082, 9780120443086, 9780080536392
ISSN
1067-2389
Open Library ID
OL18114170M
Language
english
Format
PDF
Filesize
32 MB (33262779 bytes)
Series
Methods in Molecular Genetics 6
Edition
1
Pages
3-487\507
Topic
Biology Genetics
Time added
2011-06-04 13:46:07

Description

Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages. * * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.

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