ENGLISH

Viral Gene Techniques

By Kenneth W. Adolph (Eds.)

Book information

Publisher
Elsevier, Academic Press
Year
1995
ISBN
0120443090, 9780120443093, 9780080536408
ISSN
1067-2389
Open Library ID
OL10070834M
Language
english
Format
PDF
Filesize
30 MB (31392376 bytes)
Series
Methods in Molecular Genetics 7
Edition
1
Pages
3-416\435
Topic
Biology Genetics
Time added
2011-06-04 13:46:07

Description

Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed. * Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)

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