ENGLISH

Glyco-Engineering: Methods and Protocols

By Alexandra Castilho

Book information

Publisher
Humana Press
Year
2015
ISBN
1493927590, 9781493927593
DOI
10.1007/978-1-4939-2760-9
ISSN
1064-3745
LCC
2015941740
Language
english
Format
PDF
Filesize
12 MB (12267837 bytes)
Series
Methods in Molecular Biology 1321
Pages
xvi+439\457
Topic
Biology Molecular
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-06-23 14:52:57
Protein Science

Description

Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.

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