Single Cell Biomedicine

Description

The book focuses on various detection targets applied in single cell studies, including tumor tissue cells, circulating tumor cells (CTCs), disseminated tumor cells (DTCs), circulating tumor DNA (ctDNA), cell-free DNA (cfDNA) and cancer stem cells (CSCs). It also discusses and compares detection methods using these detection targets in different fields to reveal single cell biomedical functions. The volume focuses not only on the methods already been established and validated, and also the methods newly developed. The book also highlights the importance and potential of single cell biomedicine in the development and validation of precision medicine strategies. It is useful for researchers and students in the field of cell biology, molecular medicine and precision medicine etc. Front Matter ....Pages i-vi Can the Single Cell Make Biomedicine Different? (Yuming Wang, Li Li, Xiangdong Wang, Jianqian Gu)....Pages 1-6 Automated Single-Cell Analysis and Isolation System: A Paradigm Shift in Cell Screening Methods for Bio-medicines (Kenji Tatematsu, Shun’ichi Kuroda)....Pages 7-17 Single-Cell Non-coding RNA in Embryonic Development (Qiang Fu, Chuan-Jiang Liu, Zhen-Sheng Zhai, Xu Zhang, Tao Qin, Hong-Wei Zhang)....Pages 19-32 High Throughput Single Cell RNA Sequencing, Bioinformatics Analysis and Applications (Xiaoyun Huang, Shiping Liu, Liang Wu, Miaomiao Jiang, Yong Hou)....Pages 33-43 Circulating Tumor Cells: The Importance of Single Cell Analysis (D. Heymann, M. Téllez-Gabriel)....Pages 45-58 Super-Resolution Fluorescence Microscopy for Single Cell Imaging (Han Feng, Xiaobo Wang, Zhiwei Xu, Xiaoju Zhang, Yongju Gao)....Pages 59-71 Single Cell Proteomics for Molecular Targets in Lung Cancer: High-Dimensional Data Acquisition and Analysis (Zheng Wang, Xiaoju Zhang)....Pages 73-87 Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis (Alexandria Voigt, Touyana Semenova, Janet Yamamoto, Veronique Etienne, Cuong Q. Nguyen)....Pages 89-102 Single Cell Genetics and Epigenetics in Early Embryo: From Oocyte to Blastocyst (Yubao Wei, Helong Zhang, Qian Wang, Cuilian Zhang)....Pages 103-117 The Potential Roles and Advantages of Single Cell Sequencing in the Diagnosis and Treatment of Hematological Malignancies (Mingyue Shi, Xiaoyan Dong, Lei Huo, Xiaobin Wei, Fang Wang, Kai Sun)....Pages 119-133 Application of Single Cell Sequencing in Cancer (Lan Yu, Hua Zhao, Li Meng, Cuilian Zhang)....Pages 135-148 Emergence of Bias During the Synthesis and Amplification of cDNA for scRNA-seq (Qiankun Luo, Hui Zhang)....Pages 149-158 Detection and Application of RNA Editing in Cancer (Mengjia Qian, Claudio Spada, Xiangdong Wang)....Pages 159-170 Is Pooled CRISPR-Screening the Dawn of a New Era for Functional Genomics (Jufang Yao, Hui-Li Dai)....Pages 171-176 Roles of Single Cell Systems Biomedicine in Lung Diseases (Yiming Zeng, Xiaoyang Chen, Xiangdong Wang)....Pages 177-185 The Significance of Single-Cell Biomedicine in Stem Cells (Weishan Zhuge, Furong Yan, Zhitu Zhu, Xiangdong Wang)....Pages 187-195