Citrus Tristeza Virus: Methods and Protocols

Description

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease. Front Matter ....Pages i-x A Short Note on Reflections and Publications on Citrus tristeza virus (CTV) Methodologies (Moshe Bar-Joseph)....Pages 1-6 A Brief Historical Account of the Family Closteroviridae (Giovanni P. Martelli)....Pages 7-13 Phenotyping Biological Properties of CTV Isolates (Marcella Russo, Antonino F. Catara)....Pages 15-27 CTV Vectors and Interactions with the Virus and Host Plants (Raymond Yokomi)....Pages 29-53 Tissue-Print and Squash Capture Real-Time RT-PCR Method for Direct Detection of Citrus tristeza virus (CTV) in Plant or Vector Tissues (Mariano Cambra, Eduardo Vidal, Carmen Martínez, Edson Bertolini)....Pages 55-66 Detection of Citrus tristeza virus and Coinfecting Viroids (Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, Francesco Di Serio)....Pages 67-78 Assessment of Genetic Variability of Citrus tristeza virus by SSCP and CE-SSCP (Elisavet K. Chatzivassiliou, Grazia Licciardello)....Pages 79-104 Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus tristeza virus (Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, Raymond Yokomi)....Pages 105-126 Genotyping Citrus tristeza virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-on-Chip Device (Giuseppe Scuderi, Antonino F. Catara, Grazia Licciardello)....Pages 127-142 Rapid and Sensitive Detection of Citrus tristeza virus Using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay (Dilip Kumar Ghosh, Ashish Warghane, Kajal Kumar Biswas)....Pages 143-150 Amplification and Cloning of Large cDNA Fragments of the Citrus tristeza virus Genome (Munir Mawassi, Sabrina Haviv, Ludmila Maslenin)....Pages 151-161 Bioinformatic Tools and Genome Analysis of Citrus tristeza virus (Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, Hans Jacob Maree)....Pages 163-178 Analysis of Genotype Composition of Citrus tristeza virus Populations Using Illumina Miseq Technology (David A. Read, Gerhard Pietersen)....Pages 179-194 Citrus tristeza virus: Host RNA Silencing and Virus Counteraction (Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio et al.)....Pages 195-207 Proteomic Response of Host Plants to Citrus tristeza virus (Milena Santos Dória, Carlos Priminho Pirovani)....Pages 209-218 Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment (Yosvanis Acanda, Chunxia Wang, Amit Levy)....Pages 219-228 Methods for Producing Transgenic Plants Resistant to CTV (Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores et al.)....Pages 229-243 Back Matter ....Pages 245-247