ENGLISH

Basic Cloning Procedures

By Juris Steinbergs , Aleksander Tsimanis (auth.) , Professor Dr. Valdis Berzins (eds.)

Book information

Publisher
Springer-Verlag Berlin Heidelberg
Year
1998
ISBN
978-3-642-48977-8, 978-3-642-71965-3
DOI
10.1007/978-3-642-71965-3
Language
english
Format
PDF
Filesize
3 MB (3443458 bytes)
Series
Springer Lab Manual
Edition
1
Pages
169\174
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Biochemistrygeneral; Cell Biology

Description

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

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