ENGLISH

Heterologous Protein Production in CHO Cells: Methods and Protocols

By Paula Meleady (eds.)

Book information

Publisher
Humana Press
Year
2017
ISBN
978-1-4939-6971-5, 978-1-4939-6972-2
DOI
10.1007/978-1-4939-6972-2
Language
english
Format
PDF
Filesize
6 MB (6182851 bytes)
Series
Methods in Molecular Biology 1603
Edition
1
Pages
X, 252\256
Time added
2017-06-25 01:07:36
Protein Science

Description

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.

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