Lipases and Phospholipases: Methods and Protocols

Description

This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes. Front Matter ....Pages i-xiv Front Matter ....Pages 1-1 Lipases: An Overview (Leticia Casas-Godoy, Francisco Gasteazoro, Sophie Duquesne, Florence Bordes, Alain Marty, Georgina Sandoval)....Pages 3-38 Carbohydrate Esterases: An Overview (Mariana Armendáriz-Ruiz, Jorge Alberto Rodríguez-González, Rosa María Camacho-Ruíz, Juan Carlos Mateos-Díaz)....Pages 39-68 Phospholipases: An Overview (Ahmed Aloulou, Renaud Rahier, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham)....Pages 69-105 Front Matter ....Pages 107-107 Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates (Dolores Reyes-Duarte, Cristina Coscolín, Mónica Martínez-Martínez, Manuel Ferrer, Humberto García-Arellano)....Pages 109-117 A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates (Meddy El Alaoui, Laurent Soulère, Alexandre Noiriel, Priscila Sutto-Ortiz, Lucie Grand, Florence Popowycz et al.)....Pages 119-128 Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline (Renaud Rahier, Houda Abla, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham)....Pages 129-138 Lipase and Phospholipase Activity Methods for Marine Organisms (H. Nolasco-Soria, F. Moyano-López, F. Vega-Villasante, Alberto del Monte-Martínez, D. Espinosa-Chaurand, E. Gisbert et al.)....Pages 139-167 Functional Expression of Plant Lipases: The Case of CpLip1 from Carica papaya (Ivanna Rivera, Abel Gutiérrez-Ortega, Georgina Sandoval)....Pages 169-178 Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2 (Nabil Smichi, Neila Achouri, Alexandre Noiriel, Vincent Arondel, Abdelkarim Abousalham, Youssef Gargouri et al.)....Pages 179-189 Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies (Yani Arhab, Renaud Rahier, Alexandre Noiriel, Mickael V. Cherrier, Abdelkarim Abousalham)....Pages 191-201 Front Matter ....Pages 203-203 Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review (Francisco Valero)....Pages 205-216 Solid-State Fermentation as an Economic Production Method of Lipases (Doddy Denise Ojeda-Hernández, Ricardo Cosío-Cuadros, Georgina Sandoval, Jorge Alberto Rodríguez-González, Juan Carlos Mateos-Díaz)....Pages 217-228 Synthetic Biology to Improve the Production of Lipases and Esterases (Review) (Heber Gamboa-Melendez, Macarena Larroude, Young Kyoung Park, Pauline Trebul, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro)....Pages 229-242 Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases (Alberto del Monte-Martínez, Bessy V. Cutiño-Avila, Jorge González-Bacerio)....Pages 243-283 Front Matter ....Pages 285-285 Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides (Jose L. Gonzalez-Alfonso, Leticia Casas-Godoy, Javier Arrizon, Daniel Arrieta-Baez, Antonio O. Ballesteros, Georgina Sandoval et al.)....Pages 287-296 Targeting Phospholipase D Genetically and Pharmacologically for Studying Leukocyte Function (Julian Gomez-Cambronero, Ramya Ganesan)....Pages 297-314 Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale (Suzana Ferreira-Dias, Natália M. Osório, Carla Tecelão)....Pages 315-336 Lipase-Catalyzed Acetylation and Esterification of Bile Acids (Alicia Baldessari, Guadalupe García Liñares)....Pages 337-350 Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents (Nadia Guajardo, Pablo Domínguez de María)....Pages 351-357 Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases (Alicia Baldessari, Guadalupe García Liñares)....Pages 359-376 Lipases as Biocatalyst for Biodiesel Production (Marcos Vargas, Xochitl Niehus, Leticia Casas-Godoy, Georgina Sandoval)....Pages 377-390 Lipase, Phospholipase, and Esterase Biosensors (Review) (Georgina Sandoval, Enrique J. Herrera-López)....Pages 391-425 Back Matter ....Pages 427-437