Clinical Applications of Capillary Electrophoresis: Methods and Protocols

Description

This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research. Front Matter ....Pages i-xii Front Matter ....Pages 1-1 An Overview of CE in Clinical Analysis (David S. Hage)....Pages 3-11 Front Matter ....Pages 13-13 Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies (Petr Tůma)....Pages 15-24 Monitorization of α1-Acid Glycoprotein Deglycosylation Using SU-8 Microchips Electrophoresis with LIF Detection (María del Mar Barrios-Romero, Agustín G. Crevillén, Angel Puerta, Mercedes de Frutos, José Carlos Diez-Masa)....Pages 25-39 Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis Using Electrophoretic Injection (Chenhua Zhang, William Clarke, David S. Hage)....Pages 41-56 On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin (Roger Pero-Gascon, Laura Pont, Victoria Sanz-Nebot, Fernando Benavente)....Pages 57-76 Measurement of Neutral and Sialylated IgG n-Glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis (Christian Schwedler, Véronique Blanchard)....Pages 77-93 The Control of Glucose and Lactate Levels in Nutrient Medium After Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection (Petr Tůma)....Pages 95-108 Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization (Morten E. Pedersen, Jesper Østergaard, Henrik Jensen)....Pages 109-123 Front Matter ....Pages 125-125 A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs Using Short End Injection Capillary Electrophoresis: Application to Design of Experiment (Hassan Y. Aboul-Enein, Ahmed M. Abdel-Megied)....Pages 127-136 Front Matter ....Pages 137-137 New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS) (Meera Shanmuganathan, Philip Britz-McKibbin)....Pages 139-163 Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Samples (Wei Zhang, Thomas Hankemeier, Rawi Ramautar)....Pages 165-172 Front Matter ....Pages 173-173 Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis (Anna V. Nielsen, Adam T. Woolley)....Pages 175-184 Analysis of Inflammatory Mediators in Newborn Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis (Terry M. Phillips, Edward F. Wellner)....Pages 185-198 Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions (Indhu-Shree Rajan-Babu, Samuel S. Chong)....Pages 199-210 Front Matter ....Pages 211-211 A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood (Angelo Zinellu, Elisabetta Sotgiu, Salvatore Sotgia, Ciriaco Carru)....Pages 213-219 Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA) (Noemi Farina-Gomez, Diana Navarro-Calderon, Angel Puerta, Monica Gonzalez, José Carlos Diez-Masa, Mercedes de Frutos)....Pages 221-234 Prostate Protein n-Glycosylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis (Tijl Vermassen, Nico Callewaert, Sylvie Rottey, Joris R. Delanghe)....Pages 235-250 Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen (Shulin Zhao)....Pages 251-259 Front Matter ....Pages 261-261 Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins (Chao-Xuan Zhang, Michael M. Meagher)....Pages 263-270 Back Matter ....Pages 271-273