ENGLISH

From Proteins to Proteomics: Basic Concepts, Techniques, and Applications

By Sanjeeva Srivastava

Book information

Publisher
CRC Press
Year
2022
ISBN
0367566206, 9780367566203
Language
english
Format
PDF
Filesize
40 MB (41436028 bytes)
Pages
271\272
Time added
2022-11-12 01:59:44

Description

Proteomics aims to study all the proteins of human and other living systems, as well as their properties to provide an integrated view of cellular processes. The study of proteomics involves the application of rapidly evolving high-throughput technologies and new platforms that are coming forward regularly, providing versatile novel tools for biomedical and pharmaceutical applications. This book provides a detailed understanding of the basics of proteins and proteomics, gel based-proteomics techniques, basics of mass spectrometry and quantitative proteomics, interactomics: basics and applications, and advancements in proteomics. It also covers basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. The text highlights the application and challenges of various high-throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules, which are found to be very effective for studying disease pathobiology and identification of next-generation biomarkers and potential drug/vaccine targets; and are therefore considered valuable tools for multidisciplinary research. Features Basics of proteins and proteomics techniques In-depth understanding of mass spectrometry and quantitative proteomics An overview of interactomics and its application for translational research Advancement in the field of proteomics and challenges in clinical applications We hope the knowledge gained from reading this book will intrigue and motivate young minds to explore future opportunities in the constantly evolving field of proteomics. Cover Half Title Title Page Copyright Page Contents Preface Author Module I: Basics of Proteins and Proteomics 1. Basics of Amino Acids and Proteins 2. Protein Chemistry to Proteomics Module II: Gel-Based Proteomics Techniques 3. Gel-Based Proteomics 4. 2D Difference Gel Electrophoresis (2D-DIGE) 5. Gel-Based Proteomic Data Analysis Module III: Basics of Mass Spectrometry and Quantitative Proteomics 6. Introduction to Mass Spectrometry 7. Hybrid Mass Spectrometry Configurations 8. Tandem Mass Spectrometry for Protein Identification 9. In Vitro Quantitative Proteomics Using iTRAQ 10. In Vivo Quantitative Proteomics Using SILAC Module IV: Interactomics: Basics and Application 11. Introduction to Interactomics 12. Antigen and Antibody Microarrays 13. Cell-Free Expression-Based Protein Microarrays 14. Nucleic Acid Programmable Protein Arrays 15. Label-Free Proteomics 16. Surface Plasmon Resonance 17. Surface Plasmon Resonance Imaging 18. Protein Interaction Analysis Using SPR and SPRi Module V: Advancement in Proteomics 19. Proteomics for Translational Research 20. Future of Proteomics for Clinical Applications 21. Challenges in Clinical Proteomics Index

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