ENGLISH

Protein Structure Analysis: Preparation, Characterization, and Microsequencing

By D. A. Kyriakidis (auth.) , Professor Dr. Roza Maria Kamp , Ass. Professor Dr. Theodora Choli-Papadopoulou , Professor Brigitte Wittmann-Liebold (eds.)

Book information

Publisher
Springer-Verlag Berlin Heidelberg
Year
1997
ISBN
978-3-642-47765-2, 978-3-642-59219-5
DOI
10.1007/978-3-642-59219-5
Language
english
Format
PDF
Filesize
6 MB (6055407 bytes)
Series
Springer Lab Manual
Edition
1
Pages
316\310
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Biochemistrygeneral; Cell Biology

Description

The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories; they are easy to perform and thus also suitable for beginners. Enclosed are protocols on the separation of proteins and peptides, manual and automated microsequencing, electrophoresis and blotting, analysis of amino acids, identification of cysteine residues and lipids as well as on mass spectrometry crystallization of macromolecules.

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