ENGLISH

Calpain Methods and Protocols

By Valery F Thompson , Darrel E Goll (auth.) , John S. Elce (eds.)

Book information

Publisher
Humana Press
Year
2000
ISBN
9780896036321, 0896036324
DOI
10.1385/1592590500
Open Library ID
OL16971887M
Language
english
Format
PDF
Filesize
2 MB (1651705 bytes)
Series
Methods in Molecular Biology 144
Edition
1
Pages
342\291
Topic
Biology
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Neurosciences

Description

In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of µ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.

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