3D Sponge-Matrix Histoculture

Description

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam® histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam® histoculture of skin as well as isolated hair follicles in Gelfoam® histoculture; the use of Gelfoam® histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® histoculture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting–edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture. Front Matter ....Pages i-xviii In Memoriam: Joseph Leighton, 1921–1999—Father of 3-Dimensional Tissue Culture (Robert M. Hoffman)....Pages 1-9 3D Sponge-Matrix Histoculture: An Overview (Robert M. Hoffman)....Pages 11-17 In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture (Robert M. Hoffman, Aaron E. Freeman)....Pages 19-28 Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology (Robert M. Hoffman, Fiorella Guadagni)....Pages 29-37 Development of the Histoculture Drug Response Assay (HDRA) (Robert M. Hoffman, Robert A. Vescio)....Pages 39-48 Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture (Robert M. Hoffman)....Pages 49-60 Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer (Robert M. Hoffman)....Pages 61-72 Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer (Robert M. Hoffman, Phill-Seung Jung, Moon-Bo Kim, Joo-Hyun Nam)....Pages 73-81 Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer (Robert M. Hoffman)....Pages 83-92 Clinical Usefulness of the Histoculture Drug Response Assay for Breast Cancer (Robert M. Hoffman, Hirokazu Tanino)....Pages 93-100 Clinical Usefulness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH) (Robert M. Hoffman)....Pages 101-107 In Vivo-Like Cell-Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real Time by FUCCI Imaging (Robert M. Hoffman, Shuya Yano)....Pages 109-123 Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture (Robert M. Hoffman)....Pages 125-131 Hair-Shaft Growth in Gelfoam® Histoculture of Skin and Isolated Hair Follicles (Robert M. Hoffman, Lingna Li, Wenluo Cao)....Pages 133-144 Hair Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair (Fang Liu, Robert M. Hoffman)....Pages 145-162 Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid (Robert M. Hoffman, Sumiyuki Mii, Jennifer Duong, Yasuyuki Amoh)....Pages 163-186 Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam® (Andrea Introini, Wendy Fitzgerald, Christophe Vanpouille, Leonid Margolis)....Pages 187-197 Imaging DNA Repair After UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture (Shinji Miwa, Robert M. Hoffman)....Pages 199-203 Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel (Yasunori Tome, Fuminari Uehara, Fuminori Kanaya, Robert M. Hoffman)....Pages 205-214 Imaging the Governing Step of Metastasis in Gelfoam® Histoculture (Robert M. Hoffman, Takashi Chishima)....Pages 215-220 Back Matter ....Pages 221-232