MicroRNA Protocols

Description

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that’s cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses. Front Matter ....Pages i-xi The MicroRNA (Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin)....Pages 1-25 Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro (Mainak Bose, Suvendra N. Bhattacharyya)....Pages 27-39 Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection (Anna Majer, Stephanie A. Booth)....Pages 41-51 Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid (Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma)....Pages 53-63 Profiling of MicroRNAs in the Biofluids of Livestock Species (Jason Ioannidis, Judith Risse, F. Xavier Donadeu)....Pages 65-77 Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders (Gabriel R. Fries, Joao Quevedo)....Pages 79-85 Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation (Max Hübner, Pedro A. F. Galante, Simone Kreth, Ludwig Christian Hinske)....Pages 87-92 How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and Their Target/Pathway Prediction in Bovine Ovarian Cells (Anna E. Zielak-Steciwko, John A. Browne)....Pages 93-105 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs (Shi-Lung Lin, Shao-Yao Ying)....Pages 107-126 Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration (Sang-Ging Ong, Won Hee Lee, Yang Zhou, Joseph C. Wu)....Pages 127-136 Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime (Nuno Bastos, Sónia A. Melo)....Pages 137-143 Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets (Yoichi Robertus Fujii)....Pages 145-157 In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion, and Metastasis (Jian Xu, Xuelian Xiao, Daheng Yang)....Pages 159-171 Isolation and Identification of Gene-Specific MicroRNAs (Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying)....Pages 173-180 Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells (Masashi Fukuoka, Hirohiko Hohjoh)....Pages 181-192 Screening miRNA for Functional Significance by 3D Cell Culture System (Bo Han)....Pages 193-201 Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation (Lichan Tao, Yihua Bei, Yongqin Li, Junjie Xiao)....Pages 203-213 Gene Manipulation with Micro RNAs at Single-Human Cancer Cell (Andres Stucky, Xuelian Chen, Jiang F. Zhong)....Pages 215-223 Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis (Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro et al.)....Pages 225-237 Transgene-Like Animal Models Using Intronic MicroRNAs (Shi-Lung Lin, Shin-Ju E. Chang, Shao-Yao Ying)....Pages 239-254 Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming (Zhonghui Zhang, Wen-Shu Wu)....Pages 255-263 Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction (Shi-Lung Lin, Shao-Yao Ying)....Pages 265-282 The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms (Shao-Yao Ying, William Fang, Shi-Lung Lin)....Pages 283-304 Identification and Isolation of Novel Sugar-Like RNA Protecting Materials: Glycylglycerins from Pluripotent Stem Cells (Shi-Lung Lin)....Pages 305-316 Back Matter ....Pages 317-319