ENGLISH

Transcription Factor Protocols

By Dennis K. Watson , Richard Kitching , Calvin Vary (auth.) , Martin J. Tymms (eds.)

Book information

Publisher
Humana Press
Year
2000
ISBN
9780896035737, 0896035735
DOI
10.1385/159259686X
LCC
QP552.T68 T727 2000
Open Library ID
OL371576M
Language
english
Format
PDF
Filesize
2 MB (1634239 bytes)
Series
Methods in Molecular Biology 130
Edition
1
Pages
306\285
Topic
Biology
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Human Genetics

Description

In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.

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