ENGLISH

Microsatellites: Methods and Protocols

By Panagiotis Madesis , Ioannis Ganopoulos , Athanasios Tsaftaris (auth.) , Stella K. Kantartzi (eds.)

Book information

Publisher
Humana Press
Year
2013
ISBN
1627033882, 9781627033886
DOI
10.1007/978-1-62703-389-3
ISSN
1064-3745
LCC
2013934703
Language
english
Format
PDF
Filesize
6 MB (6554869 bytes)
Series
Methods in Molecular Biology 1006
Edition
1
Pages
339\353
Topic
Biology Genetics
Orientation
portrait
Paginated
yes
Scanned
no
Time added
2013-08-07 11:50:52
Human Genetics

Description

Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.

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