Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods

Description

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Content: Series PagePage ii CopyrightPage iv ContributorsPages xi-xiv PrefacePages xv-xviMaria Spies, Yann R. Chemla Chapter One - How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-ClampPages 1-29J. Sung, K.I. Mortensen, J.A. Spudich, H. Flyvbjerg Chapter Two - Studying the Mechanochemistry of Processive Cytoskeletal Motors With an Optical TrapPages 31-54V. Belyy, A. Yildiz Chapter Three - Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a ReplisomePages 55-84B. Sun, M.D. Wang Chapter Four - Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence MicroscopyPages 85-119M. Hashemi Shabestari, A.E.C. Meijering, W.H. Roos, G.J.L. Wuite, E.J.G. Peterman Chapter Five - Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical TrappingPages 121-136C.-T. Lin, T. Ha Chapter Six - High-Resolution Optical Tweezers Combined With Single-Molecule Confocal MicroscopyPages 137-169K.D. Whitley, M.J. Comstock, Y.R. Chemla Chapter Seven - Integrating Optical Tweezers, DNA Tightropes, and Single-Molecule Fluorescence Imaging: Pitfalls and TrapsPages 171-192J. Wang, J.T. Barnett, M.R. Pollard, N.M. Kad Chapter Eight - Single-Stranded DNA Curtains for Studying Homologous RecombinationPages 193-219C.J. Ma, J.B. Steinfeld, E.C. Greene Chapter Nine - Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch RepairPages 221-238M.W. Brown, A. de la Torre, I.J. Finkelstein Chapter Ten - Single-Molecule Insight Into Target Recognition by CRISPR–Cas ComplexesPages 239-273M. Rutkauskas, A. Krivoy, M.D. Szczelkun, C. Rouillon, R. Seidel Chapter Eleven - Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single MoleculesPages 275-296C. Duboc, J. Fan, E.T. Graves, T.R. Strick Chapter Twelve - Measuring Force-Induced Dissociation Kinetics of Protein Complexes Using Single-Molecule Atomic Force MicroscopyPages 297-320K. Manibog, C.F. Yen, S. Sivasankar Chapter Thirteen - Improved Force Spectroscopy Using Focused-Ion-Beam-Modified CantileversPages 321-351J.K. Faulk, D.T. Edwards, M.S. Bull, T.T. Perkins Chapter Fourteen - Single-Molecule Characterization of DNA–Protein Interactions Using Nanopore BiosensorsPages 353-385A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul, A. Meller Chapter Fifteen - Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNTPages 387-414A.H. Laszlo, I.M. Derrrington, J.H. Gundlach Chapter Sixteen - Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme DynamicsPages 415-435S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap, L. Finzi Author IndexPages 437-458 Subject IndexPages 459-467