Handbook of Biological Confocal Microscopy

Description

Front Matter....Pages i-xiii Foundations of Confocal Scanned Imaging in Light Microscopy....Pages 1-14 Fundamental Limits in Confocal Microscopy....Pages 15-26 Quantitative Fluorescence Imaging with Laser Scanning Confocal Microscopy....Pages 27-39 The Pixelated Image....Pages 41-51 Laser Sources for Confocal Microscopy....Pages 53-67 Non-laser Illumination for Confocal Microscopy....Pages 69-76 Objective Lenses For Confocal Microscopy....Pages 77-86 Size and Shape of The Confocal Spot: Control and Relation to 3D Imaging and Image Processing....Pages 87-91 The Intermediate Optical System of Laser-scanning Confocal Microscopes....Pages 93-103 Intermediate Optics in Nipkow Disk Microscopes....Pages 105-111 The Role of the Pinhole in Confocal Imaging Systems....Pages 113-126 Photon Detectors for Confocal Microscopy....Pages 127-139 Manipulation, Display, and Analysis of Three-Dimensional Biological Images....Pages 141-150 Three-dimensional Imaging on Confocal and Wide-field Microscopes....Pages 151-161 Direct Recording of Stereoscopic Pairs Obtained From Disk-scanning Confocal Light Microscopes....Pages 163-168 Fluorophores for Confocal Microscopy: Photophysics and Photochemistry....Pages 169-178 Image Contrast in Confocal Light Microscopy....Pages 179-195 Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy....Pages 197-205 A Comparison of Various Optical Sectioning Methods: The Scanning Slit Confocal Microscope....Pages 207-214 Back Matter....Pages 215-232