Protein Liquid Chromatography

Description

Paperback. This book is about liquid chromatography as a tool for isolating and purifying proteins and appears as a handbook-wise linear selection of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase. Part B deals with various target protein classes like membrane proteins, recombinant proteins, and glycoproteins. Finally, Part C looks at various miscellaneous, mutual aspects including coupling reactions, buffer solution additives, and software. The book as a whole can be viewed as a systematic survey of what can be utilized and optimized these days but it also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the essence of the whole contribution. It has been considered very important in this compilation to adhere to the philosophy that practical application is ever Content: Edited by Page iii Copyright page Page iv Preface Pages v-vi Michael Kastiwr List of Contributors Pages vii-x Overview of Chapterers Page xi Chapter 1 Ion Exchange Chromatography Original Research Article Pages 3-88 Peter H. Roos Chapter 2 Size Exclusion Chromatography Original Research Article Pages 89-145 Matthias Rögner Chapter 3 Reversed-Phase Chromatography Original Research Article Pages 147-234 Hartmut SchlüTer Chapter 4 Hydrophobic Interaction Chromatography Original Research Article Pages 235-269 Lothar R. Jacob Chapter 5 Hydroxyapatite Chromatography Original Research Article Pages 271-299 Wolfgang R. Deppert, Richard Lukaĉin Chapter 6 Immobilized Metal Ion Afinity Chromatography Original Research Article Pages 301-383 Michael Kastner Chapter 7 Chromatofocusing Original Research Article Pages 385-414 Richard Lukaĉ, Wolfgang R. Deppert Chapter 8 Dye-Ligand Affinity Chromatography Original Research Article Pages 415-448 Jürgen Kirchberger, Hans-Joachim Böhme Chapter 9 Immobilized Artificial Membrane Chromatography Original Research Article Pages 449-468 Kimberly Hauer, Hanlan Liu, Charles Pidgeon Chapter 10 Liquid-Liquid Partition Chromatography Original Research Article Pages 469-504 Ulla-Britt Hansson, Christer Wingren Chapter 11 Displacement Chromatography Original Research Article Pages 505-524 Hartmut Schlüter, Joachim Jankowski Chapter 12 Integral Membrane Proteins Original Research Article Pages 527-556 Gjalt W. Welling, Sytske Welling-Wester Chapter 13 Recombinant Proteins Original Research Article Pages 557-586 Otmar Huber, Martina Huber-Wunderlich Chapter 14 DNA-Binding Proteins Original Research Article Pages 587-617 Mathias Velleman Chapter 15 Lectins and Glycoconjugates Original Research Article Pages 619-638 Hans-Joachim Gabius Chapter 16 Covalent Chromatography Original Research Article Pages 639-665 Donald Whitney Chapter 17 Cell Afinity Chromatography Original Research Article Pages 667-702 David D. Putnam, Barry F. Wolf, Mark A. Burns Chapter 18 Coupling Reactions Original Research Article Pages 705-716 Egbert Müller Chapter 19 Immobilization of Nucleotides Original Research Article Pages 717-729 Egbert Müller, Michael Morr Chapter 20 Miscellaneous Biospecific Affinity Gels Original Research Article Pages 731-764 Alan M. Williams Chapter 21 Scale-up of Downstream Processing Original Research Article Pages 765-783 Joachim Walter Chapter 22 Chromatography on Porous Matrices Original Research Article Pages 785-824 Jochen Kruip Chapter 23 Chromatography Using Strep-tag Affinity Peptides Original Research Article Pages 825-837 Holger N. Müler, Thomas G.M. Schmidt Chapter 24 Buffers and Additives Original Research Article Pages 839-862 Wolfgang R. Deppert, Richard Lukaĉin Chapter 25 Chromatography Software Original Research Article Pages 863-876 Thomas Steger-Hartmann Abbreviations used in this book Pages 879-885 Directory of Manufacturers and Suppliers Pages 887-894 Subject Index Pages 895-936