ENGLISH

Plant MicroRNAs: Methods and Protocols

By Zhixin Xie (auth.) , Blake C. Meyers , Pamela J. Green (eds.)

Book information

Publisher
Humana Press
Year
2010
ISBN
9781603270045, 1603270043
DOI
10.1007/978-1-60327-005-2
LCC
QK981.4 .P5797 2010
Open Library ID
OL25007981M
Language
english
Format
PDF
Filesize
8 MB (8474596 bytes)
Series
Methods in Molecular Biology 592
Edition
1
Pages
261\272
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Plant Sciences; Plant Genetics & Genomics; Gene Fu

Description

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.

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