Rare-Earth Element Biochemistry: Methanol Dehydrogenases and Lanthanide Biology
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Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more. Contents Contributors Preface 1. Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs • Akio Tani, Ryoji Mitsui, and Tomoyuki Nakagawa 2. Synthetic modeling of the structure and function of the rare-earth dependent methanol dehydrogenase cofactor • Alison L. Knasin and Eric J. Schelter 3. Activity assays of methanol dehydrogenases • Sophie M. Gutenthaler, Manh Tri Phi, Helena Singer, and Lena J. Daumann 4. Expression, purification and properties of the enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, and XoxG4 • Jing Huang, Yue Zheng, Joseph D. Groom, Zheng Yu, and Ludmila Chistoserdova 5. Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1 • Nathan M. Good and N. Cecilia Martinez-Gomez 6. Heterologous expression, purification, and characterization of proteins in the lanthanome • Emily R. Featherston, Joseph A. Mattocks, Jonathan L. Tirsch, and Joseph A. Cotruvo Jr. 7. Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system • Huong N. Vu, Gabriel A. Subuyuj, Ralph Valentine Crisostomo, and Elizabeth Skovran 8. Metals and methylotrophy: Via global gene expression studies • Zachary J. Johnson, Dennis D. Krutkin, Pavlo Bohutskyi, and Marina G. Kalyuzhnaya 9. Mass spectrometry-based approaches to study lanthanides and lanthanide-dependent proteins in the phyllosphere • Lucas Hemmerle, Andrea M. Ochsner, Thomas Vonderach, Bodo Hattendorf, and Julia A. Vorholt 10. Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph “Methylotuvimicrobium buryatense” 5GB1C • Joseph D. Groom and Mary E. Lidstrom
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