ENGLISH

Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods

By Francisco J. Pelegri

Book information

Publisher
Springer New York;Humana Press
Year
2019
ISBN
978-1-4939-9008-5, 978-1-4939-9009-2
Language
english
Format
PDF
Filesize
10 MB (10732799 bytes)
Series
Methods in Molecular Biology 1920
Edition
2nd ed.
Pages
XI, 434\434
Time added
2019-04-19 19:00:00
Life Sciences; Embryology; Animal Models; Animal G

Description

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development. Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. Front Matter ....Pages i-xi Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments (Douglas W. Houston)....Pages 1-16 Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos (Thao Nguyen, Timothy J. Mitchison, Martin Wühr)....Pages 17-32 Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos (Michael D. Sheets)....Pages 33-39 Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts (Alexander A. Tokmakov, Ken-Ichi Sato)....Pages 41-57 Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis (Ken-Ichi Sato, Alexander A. Tokmakov)....Pages 59-73 Embryological and Genetic Manipulation of Chick Development (Laura S. Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal)....Pages 75-97 Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos (Ana Paula Azambuja, Marcos Simoes-Costa)....Pages 99-110 Experimental Manipulation of Ploidy in Zebrafish Embryos and Its Application in Genetic Screens (Triveni Menon, Sreelaja Nair)....Pages 111-128 RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) (Karoline Holler, Jan Philipp Junker)....Pages 129-141 Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos (Clare E. Buckley)....Pages 143-162 Ex Utero Culture and Imaging of Mouse Embryos (Sonja Nowotschin, Vidur Garg, Anna Piliszek, Anna-Katerina Hadjantonakis)....Pages 163-182 Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections (Edwina McGlinn, Miriam A. Holzman, Jennifer H. Mansfield)....Pages 183-218 Reptile Embryology and Regeneration (Matthew K. Vickaryous, Emily A. B. Gilbert)....Pages 219-246 Manipulation of Developmental Function in Turtles with Notes on Alligators (Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, Judith A. Cebra-Thomas)....Pages 247-263 Methods for Isolating the Balbiani Body/Germplasm from Xenopus laevis Oocytes (Amanda Butler, Dawn Owens, Mary Lou King, Tristan Aguero)....Pages 265-275 Visualizing the Balbiani Body in Zebrafish Oocytes (KathyAnn L. Lee, Florence L. Marlow)....Pages 277-293 Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis (Allison Jamieson-Lucy, Mary C. Mullins)....Pages 295-302 Generation of Xenopus Haploid, Triploid, and Hybrid Embryos (Romain Gibeaux, Rebecca Heald)....Pages 303-315 Germ Cell Transplantation in Avian Species (Young Hyun Park, Young Min Kim, Jae Yong Han)....Pages 317-326 Production of Germ-Line Chimeras in Zebrafish (Taiju Saito, Rie Goto, Nicola Rivers, Etsuro Yamaha)....Pages 327-341 A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring (Zsolt Csenki, Ferenc Mueller)....Pages 343-352 Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) (Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, Jose B. Cibelli)....Pages 353-375 An Accessible Protocol for the Generation of CRISPR-Cas9 Knockouts Using INDELs in Zebrafish (Cara E. Moravec, Francisco J. Pelegri)....Pages 377-392 Modeling Embryonic Cleavage Patterns (Dmitry Ershov, Nicolas Minc)....Pages 393-406 Laboratory Guidelines for Animal Care (Marcelo Couto, Charles Cates)....Pages 407-430 Back Matter ....Pages 431-434

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