Immunosensors

Description

Expert scientists are covering a selection of high quality representative examples from the last five years explaining how immunosensors for multiple target analysis using laboratory based or point-of-care set-up have developed. Cover Immunosensors Preface Contents Chapter 1 -- Introduction to Immunosensors 1.1 Introduction 1.2 Basic Principles of an Immunosensor 1.2.1 Antibodies and Their Application to Immunosensors 1.2.2 Immunosensor Format 1.2.2.1 Competitive Immunoassay System 1.2.2.2 Direct Immunoassay 1.2.2.3 Indirect Immunoassay 1.2.2.4 Non-competitive Format (Sandwich Format) 1.3 Architectures of Transducers and Their Potential Applications 1.3.1 Electrochemical Immunosensor 1.3.1.1 Types of Electrochemical Immunosensor 1.3.1.1.1 Amperometric Immunosensor. Interest in amperometric biosensors began when it was observed that potentiostatic procedures are capa ... 1.3.1.1.2 Voltammetry. Voltammetry involves the measurement of current and potential where the potential is scanned over a pre- set potenti ... 1.3.1.1.3 Potentiometry. Potentiometric measurement records and displays the activity of ions in an electrochemical reaction. It specifies ... 1.3.1.1.4 Impedance. Electrochemical impedance spectroscopy (EIS) measures the electrical impedance of an interface by applying a small sin ... 1.3.1.2 Application of Electrochemical Immunosensors1.3.2 Optical Immunosensors 1.3.2.1 Surface Plasmon Resonance (SPR) 1.3.2.2 Performance 1.3.3 Piezoelectric Immunosensor 1.3.3.1 Types of Piezoelectric Immunosensor 1.3.3.1.1 Quartz Crystal Microbalance (QCM) Immunosensor. A quartz crystal microbalance (QCM) configuration is made up of a thin quartz pla ... 1.3.3.1.2 Surface Wave Acoustic Immunosensor. A surface wave acoustic (SAW) immunosensor device set- up usually comprises interdigitated me ... 1.3.3.2 Analytical Performance 1.3.4 Thermometric Immunosensor 1.4 Conclusions and Future OutlooksAcknowledgements References Chapter 2 -- Structure, Function, Orientation, Characterization and Immobilization of Antibodies for Immunosensor Development 2.1 Introduction 2.2 Fundamentals, Structural Feasibility and Functions of Antibodies 2.3 Immunosensor Development Using Antibodies 2.3.1 Monoclonal Antibodies 2.3.2 Polyclonal Antibodies 2.3.3 Chopped/Half-antibodies 2.3.4 Synthetic Antibodies (Aptamers/Imprinted Polymers) 2.4 Strategies Towards Immobilization of Antibodies 2.5 Characterization Techniques 2.6 Label-free and Labeled Immunosensing Techniques2.6.1 Optical Immunosensor 2.6.2 Piezoelectric Immunosensor 2.6.3 Impedimetric Immunosensor 2.6.4 Thermal Immunosensor 2.7 Recent Developments and Applications 2.8 Conclusion Acknowledgements References Chapter 3 -- Immunosensing With Electro-active Photonic Devices 3.1 Introduction 3.2 Immunosensing with Single-mode, Electro-active, Integrated Optical Waveguides 3.2.1 Methodology 3.2.2 Sample Preparation 3.2.2.1 SM-EA- IOW Fabrication