Allergy: Methods and Protocols

Description

This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interested in beginning or continuing their research in the study of allergies. Front Matter ....Pages i-x Understanding of the Molecular Mechanisms of Allergy (Meinir G. Jones)....Pages 1-15 T Cell: Primary Culture from Peripheral Blood (Monika Raulf)....Pages 17-31 Measurement of Allergen-Specific Inhibitory Antibody Activity (Mohamed H. Shamji, James N. Francis)....Pages 33-43 Immunoelectrophoresis for the Characterization of Allergen Extracts (Gitte Nordskov Hansen, Jørgen Nedergaard Larsen)....Pages 45-62 Standardization of Allergen Extracts (Jørgen Nedergaard Larsen, Sten Dreborg)....Pages 63-76 Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation (Natascha Fitch, Stephanie J. Marshall, William P. Stefura, Rishma Chooniedass, Allan B. Becker, Kent T. HayGlass)....Pages 77-89 Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays (William P. Stefura, Caroline Graham, Larisa Lotoski, Kent T. HayGlass)....Pages 91-114 Development of Mold Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure (Eva Zahradnik, Monika Raulf)....Pages 115-130 Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production (Sun-Sang J. Sung)....Pages 131-141 The Use of Dual-Cell-Tracker Dye Staining for the Identification and Characterization of Peanut-Specific T-Cell Subsets (Cecilia Dunsterville, Alick C. Stephens, Gideon Lack, Victor Turcanu)....Pages 143-152 The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells (Erik Wambre)....Pages 153-164 Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies (Nadine E. G. Upton, Thomas S. Hayday, Louisa K. James)....Pages 165-174 Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure (Daniel Álvarez-Simón, Susana Gómez-Ollés)....Pages 175-184 Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors (Jihui Zhang, Clive Robinson)....Pages 185-205 Measurement of Specific IgG Anti-Fel d 1 Antibodies (Meinir G. Jones)....Pages 207-214 Measurement of Specific IgG4 Anti-mouse Urine Antibodies (Jennifer Canizales)....Pages 215-221 Preparation of Samples for a Mass Spectrometry-Based Method to Identify Allergenic Proteins (Mary Brian)....Pages 223-237 Back Matter ....Pages 239-240