Yersinia Pestis Protocols

Description

This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields. Front Matter ....Pages i-vi Isolation and Identification of Yersinia pestis (Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang et al.)....Pages 1-14 Isolation and Characterization of Yersinia pestis Bacteriophages (Xiangna Zhao)....Pages 15-22 Extraction of Chromosomal DNA and Plasmid from Yersinia pestis (Yazhou Zhou)....Pages 23-27 Extraction of Total RNA from Yersinia pestis (Haili Wang, Yanping Han)....Pages 29-34 Genotyping of Yersinia pestis (Yanjun Li, Yujun Cui)....Pages 35-59 Genome-Wide Variation Analysis of Yersinia pestis (Chao Yang, Yujun Cui)....Pages 61-66 Genome-Wide Detection of Expressed sRNAs in Yersinia pestis with RNA-seq (Yanping Han)....Pages 67-80 Validation of Yersinia pestis sRNAs with Nonradioactive-Labeled Northern Blotting (Yanping Han)....Pages 81-88 Determination of Transcription Start Sites (TSSs) in Yersinia pestis with a Primer Extension Assay (Yanping Han)....Pages 89-98 Purification of Hfq-Associated RNAs with RNA Immunoprecipitation (RIP) (Yanping Han)....Pages 99-106 Overexpression of Target sRNAs in Tightly Controlled Multicopy Plasmids (Xiaofang Gao, Zizhong Liu, Yanping Han)....Pages 107-113 Target Validation of sRNA with a GFP Reporter Gene Fusion System (Xiaofang Gao, Zizhong Liu, Yanping Han)....Pages 115-120 Inducible Gene Expression in Yersinia pestis (Juping Zhao, Yicheng Sun)....Pages 121-126 Analysis of Yops Secretion by Yersinia pestis Type III Secretion System (Zongmin Du, Shiyang Cao)....Pages 127-132 Co-immunoprecipitation Analysis for the Detection of Protein–Protein Interactions in Yersinia pestis (Shiyang Cao, Zongmin Du)....Pages 133-138 Detection of Type III Secretion System Needle Assembly in Yersinia pestis by Cross-Linking YscF (Shiyang Cao, Zongmin Du)....Pages 139-143 Evaluation of Protein Translocation into Eukaryotic Cells by Yersinia pestis (Yafang Tan)....Pages 145-150 Adhesion Analysis of Yersinia pestis to Host Cells (Yafang Tan)....Pages 151-156 Construction of Gene Deletion Mutants in Yersinia pestis (Wenliang Li, Dan Rong, Yanping Han)....Pages 157-163 CRISPR-Cas12a-Assisted Recombineering in Yersinia pestis (Juping Zhao, Yicheng Sun)....Pages 165-172 Plasmid Curing in Yersinia pestis (Yazhou Zhou)....Pages 173-182 Genetic Regulation Analysis of Yersinia pestis (Yiquan Zhang)....Pages 183-202 Investigation of Biofilm Formation in Yersinia pestis (Haihong Fang)....Pages 203-212 Extraction and Purification of F1 Capsule Antigen from Y. pestis (Xiaoyi Wang)....Pages 213-219 Screening of Regulator Responses to Yersinia pestis Infection with a Concatenated Tandem Array of Transcription Factor Response Element (catTFRE) Pulldown (Chune Jing)....Pages 221-230 Animal Models for Yersinia pestis (Yujing Bi, Xiaoyi Wang)....Pages 231-236 Antibiotic Resistance Evaluation of Yersinia pestis (Jingyu Guo)....Pages 237-242 PCR for Detection and Identification of Yersinia pestis (Yong Zhao)....Pages 243-249 Immunological Methods for Detection and Identification of Yersinia pestis (Yong Zhao)....Pages 251-257 Analysis of Fatty Acids in Yersinia pestis by Gas Chromatography (Yafang Tan)....Pages 259-265 Evaluation of Efficacy of Yersinia pestis Vaccines (Xiaoyi Wang)....Pages 267-272 Online Resources for Yersinia pestis (Yanfeng Yan)....Pages 273-279 Effects of Y. pestis on Macrophages (Yujing Bi)....Pages 281-290